2. Signaling Pathways
  3. Cell Cycle/DNA Damage
  4. Poly(ADP-ribose) Glycohydrolase (PARG)

Poly(ADP-ribose) Glycohydrolase (PARG)

Poly (ADP-ribose) glycohydrolase (PARG) is the primary hydrolase involved in the degradation of poly(ADP-ribose) (PAR). PARG possesses both endo-glycohydrolase and exo-glycohydrolase activity, preferentially performing the latter by binding to the two most distal ADP-ribose residues within the PAR chain. These different modes of catalysis produce free PAR and mono ADP-ribose moieties, respectively. The free mono ADP-ribose is then metabolized into AMP and ribose 5′ phosphate by ADP-ribose pyrophosphohydrolases such as the NUDIX family. AMP is utilized in ATP reformation and different metabolic and cell signaling pathways while ribose 5′ phosphate is a precursor to many biomolecules including DNA, RNA and ATP. Endo-glycohydrolase activity is considered to occur primarily during hyper-PARP activation, the resulting free PAR chains produced are then implicated in apoptosis acting as a death signal.

Cat. No. Product Name Effect Purity Chemical Structure
  • HY-171789
    PARG-IN-7
    		Inhibitor
    PARG-IN-7 (Example 38) is a Poly ADP-ribose glycohydrolase (PARG) inhibitor (IC50: < 0.1 μM). PARG-IN-7 inhibits cell viability of HCC1806-XRCC1 KD (knock down) cells with an IC50 < 1 μM. PARG-IN-7 can be used for cancer research.
    PARG-IN-7
  • HY-177631
    Etidaligide
    		Inhibitor
    Etidaligide, an AsiDNA, a first-in-class DNA repair inhibitor designed to prevent the repair of DNA damage in tumour cells. It also activates DNA-dependent protein kinase (DNA-PK) and poly (ADP-ribose) polymerase enzymes that induce phosphorylation of H2A
    Etidaligide
  • HY-146248
    TFMU-ADPr
    		Substrate
    TFMU-ADPr is a selective reporter substrate of SARS-CoV-2 Macro1 (IC50=0.59 μM), with an excitation wavelength (λEx) of 385 nm, and an emission wavelength (λEm) of 502 nm (or 495 nm). TFMU-ADPr can also undergo enzymatic hydrolysis with Poly(ADP-ribose) Glycohydrolase (PARG) sourced from human, Tetrahymena thermophila and ADP-ribosylhydrolase 3 from human to release fluorophores, thereby directly reporting total poly (ADP-ribose) hydrolase activity. TFMU-ADPr binds to the ADPr-binding site of SARS-CoV-2 Macro1, and its TFMU moiety inserts into the narrow hydrophobic groove of this protein. TFMU-ADPr can thus be used to evaluate small-molecule inhibitors targeting PAR hydrolases under in vitro conditions, to investigate the regulatory mechanisms of ADP-ribosyl catabolic enzymes, or to detect PAR hydrolase activity in whole-cell lysate assays. TFMU-ADPr is also applicable to COVID-19-related research.
    TFMU-ADPr
  • HY-177631A
    Etidaligide sodium
    		Inhibitor
    Etidaligide sodium, an AsiDNA, a first-in-class DNA repair inhibitor designed to prevent the repair of DNA damage in tumour cells. It also activates DNA-dependent protein kinase (DNA-PK) and poly (ADP-ribose) polymerase enzymes that induce phosphorylation
    Etidaligide sodium
  • HY-134354
    pNP-ADPr
    pNP-ADPr is a colorimetric substrate that used for the first continuous Poly(ADP-ribose) glycohydrolase (PARG) and ADP-ribosyl hydrolase 3 (ARH3) activity assays. pNP-ADPr can be used for the research of poly(ADP-ribose)polymerase (PARP) enzymes.
    pNP-ADPr
  • HY-164757
    PARP-1-IN-32
    		Inhibitor
    PARP-1-IN-32 (compound 15) is a poly (ADPribose) polymerase-1 (PARP-1) inhibitor. PARP-1-IN-32 can be used in cancer research.
    PARP-1-IN-32
  • HY-108360R
    PDD 00017273 (Standard)
    PDD 00017273 (Standard) is the analytical standard of PDD 00017273 (HY-108360). This product is intended for research and analytical applications. PDD 00017273 is a potent inhibitor of Poly(ADP-ribose) Glycohydrolase (PARG), with an IC50 of 26 nM, and a Kd of 1.45 nM.
    PDD 00017273 (Standard)
Cat. No. Product Name / Synonyms Application Reactivity