BDP 581/591 NHS ester

Cat. No.: HY-D1653 Purity: 98.92%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

BDP 581/591 NHS ester is a a borondipyrromethene dye (Ex=585 nm, Em=594 nm) that has relatively long fluorescence lifetime and two photon excitation cross section. BDP 581/591 NHS ester can be used for fluorescence polarization analysis and also reacts with reactive oxygen species (ROS) and alter fluorescence. BDP 581/591 NHS ester is also an NHS ester derivative that can be used to bind primary and secondary amine groups of proteins, peptides and other molecules.

For research use only. We do not sell to patients.

BDP 581/591 NHS ester Chemical Structure

CAS No. : 654651-21-5

Size	Price	Stock	Quantity
1 mg	USD 100	In-stock	Estimated Time of Arrival: December 31
5 mg	USD 300	In-stock	Estimated Time of Arrival: December 31
10 mg		Get quote
50 mg		Get quote

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Based on 1 publication(s) in Google Scholar

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Purity & Documentation
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Description

Molecular Weight

489.28

Formula

C₂₆H₂₂BF₂N₃O₄

CAS No.

654651-21-5

Appearance

Solid

Color

Brown to black

Emission (Em)

591

Excitation (Ex)

581

SMILES

[F-][B+3]1([N-]2C(C=C3[N]1=CC(/C=C/C=C/C4=CC=CC=C4)=C3)=CC(CCC(ON5C(CCC5=O)=O)=O)=C2)[F-]

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility

In Vitro:

DMF : 50 mg/mL (102.19 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.0438 mL	10.2191 mL	20.4382 mL
5 mM	0.4088 mL	2.0438 mL	4.0876 mL
10 mM	0.2044 mL	1.0219 mL	2.0438 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

In Vivo Dissolution Calculator

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Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: 98.92%

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Data Sheet (242 KB) SDS (251 KB)

COA (247 KB) HNMR (273 KB) RP-HPLC (245 KB) MS (69 KB)

Handling Instructions (2659 KB)

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMF	1 mM	2.0438 mL	10.2191 mL	20.4382 mL	51.0955 mL
	5 mM	0.4088 mL	2.0438 mL	4.0876 mL	10.2191 mL
	10 mM	0.2044 mL	1.0219 mL	2.0438 mL	5.1095 mL
	15 mM	0.1363 mL	0.6813 mL	1.3625 mL	3.4064 mL
	20 mM	0.1022 mL	0.5110 mL	1.0219 mL	2.5548 mL
	25 mM	0.0818 mL	0.4088 mL	0.8175 mL	2.0438 mL
	30 mM	0.0681 mL	0.3406 mL	0.6813 mL	1.7032 mL
	40 mM	0.0511 mL	0.2555 mL	0.5110 mL	1.2774 mL
	50 mM	0.0409 mL	0.2044 mL	0.4088 mL	1.0219 mL
	60 mM	0.0341 mL	0.1703 mL	0.3406 mL	0.8516 mL
	80 mM	0.0255 mL	0.1277 mL	0.2555 mL	0.6387 mL
	100 mM	0.0204 mL	0.1022 mL	0.2044 mL	0.5110 mL