Sodium Dodecyl Sulfate PolyAcrylamide Gel Electrophoresis (SDS-PAGE)

Definition:

SDS-PAGE is an electrophoresis method that allows protein separation by massbetween 5 and 250 kDa.SDS-PAGE has the full name of sodium dodecyl sulfate-polyacrylamide gel electrophoresis, the method is composed of gel preparation, sample preparation, electrophoresis,protein staining or western blotting and analysis of the generated banding pattern.

References:

[1]. Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680-5. [Content Brief]

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