2. Academic Validation
  3. DC-SIGN (CD209) mediates dengue virus infection of human dendritic cells

DC-SIGN (CD209) mediates dengue virus infection of human dendritic cells

  • J Exp Med. 2003 Apr 7;197(7):823-9. doi: 10.1084/jem.20021840.
Boonrat Tassaneetrithep 1,  Timothy H Burgess,  Angela Granelli-Piperno,  Christine Trumpfheller,  Jennifer Finke,  Wellington Sun,  Michael A Eller,  Kovit Pattanapanyasat,  Suttipant Sarasombath,  Deborah L Birx,  Ralph M Steinman,  Sarah Schlesinger,  Mary A Marovich
Affiliations

Affiliation

  • 1 Division of Retrovirology, Walter Reed Army Institite of Research and Henry M. Jackson Foundation for the Advancement of Military Medicine Diseases, Rockville, MD 20850, USA. [email protected]
PMID: 12682107 DOI: 10.1084/jem.20021840
Abstract

Dengue virus is a single-stranded, enveloped RNA virus that productively infects human dendritic cells (DCs) primarily at the immature stage of their differentiation. We now find that all four serotypes of dengue use DC-SIGN (CD209), a C-type lectin, to infect dendritic cells. THP-1 cells become susceptible to dengue Infection after transfection of DC-specific ICAM-3 grabbing nonintegrin (DC-SIGN), or its homologue L-SIGN, whereas the Infection of dendritic cells is blocked by anti-DC-SIGN Antibodies and not by Antibodies to other molecules on these cells. Viruses produced by dendritic cells are infectious for DC-SIGN- and L-SIGN-bearing THP-1 cells and other permissive cell lines. Therefore, DC-SIGN may be considered as a new target for designing therapies that block dengue Infection.

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