2. Academic Validation
  3. Identification of a novel serine/threonine kinase that inhibits TNF-induced NF-kappaB activation and p53-induced transcription

Identification of a novel serine/threonine kinase that inhibits TNF-induced NF-kappaB activation and p53-induced transcription

  • Biochem Biophys Res Commun. 2003 Oct 3;309(4):774-8. doi: 10.1016/j.bbrc.2003.08.069.
Jun Huang 1,  Lin Teng,  Ting Liu,  Lixia Li,  Danying Chen,  Fu Li,  Liang-Guo Xu,  Zhonghe Zhai,  Hong-Bing Shu
Affiliations

Affiliation

  • 1 Department of Cell Biology and Genetics, College of Life Sciences, Peking University, Beijing 100871, China.
PMID: 13679039 DOI: 10.1016/j.bbrc.2003.08.069
Abstract

SINK is a p65-interacting protein that inhibits PKAc-induced phosphorylation of p65 and NF-kappaB transcriptional competence. We identified a SINK-homologous serine/threonine kinase SHIK. SHIK is ubiquitously expressed and is localized in the cytoplasm. Overexpression of SHIK inhibits TNF-triggered NF-kappaB activation in reporter gene assays. Overexpression of SHIK also inhibits p53-mediated transcription in reporter gene assays, while a point mutant (D197-->I) of SHIK potentiates p53-mediated transcription. Our findings suggest that SHIK is a negative regulator of NF-kappaB- and p53-mediated gene transcription.

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