2. Academic Validation
  3. Human claspin is a ring-shaped DNA-binding protein with high affinity to branched DNA structures

Human claspin is a ring-shaped DNA-binding protein with high affinity to branched DNA structures

  • J Biol Chem. 2004 Sep 17;279(38):39289-95. doi: 10.1074/jbc.M405793200.
Funda Sar 1,  Laura A Lindsey-Boltz,  Deepa Subramanian,  Deborah L Croteau,  Stephanie Q Hutsell,  Jack D Griffith,  Aziz Sancar
Affiliations

Affiliation

  • 1 Department of Biochemistry and Biophysics and Lineberger Comprehensive Cancer Center, University of North Carolina School of Medicine, Chapel Hill, North Carolina 27599, USA.
PMID: 15226314 DOI: 10.1074/jbc.M405793200
Abstract

Claspin is an essential protein for the ATR-dependent activation of the DNA replication checkpoint response in Xenopus and human cells. Here we describe the purification and characterization of human Claspin. The protein has a ring-like structure and binds with high affinity to branched DNA molecules. These findings suggest that Claspin may be a component of the replication ensemble and plays a role in the replication checkpoint by directly associating with replication forks and with the various branched DNA structures likely to form at stalled replication forks because of DNA damage.

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