Antiproliferative effect of trichostatin A and HC-toxin in T47D human breast cancer cells

Histone deacetylase inhibitors are new class of chemotherapeutic drugs able to induce tumor cell Apoptosis and/or cell cycle arrest. Trichostatin A, an antifungal Antibiotic, and HC-toxin are potent and specific inhibitors of histone deacetylase activity. In this study, we have examined the antiproliferative activities of trichostatin A and HC-toxin in Estrogen Receptor positive human breast Cancer, T47D cells. Both trichostatin A and HC-toxin showed potent antiproliferative efficacy and cell cycle arrest at G2/M in T47D human breast Cancer cells in a dose-dependent manner. Trichostatin A caused potent Apoptosis of T47D human breast Cancer cells and trichostatin A-induced Apoptosis might be involved in an increase of Caspase-3/7 activity. HC-toxin evoked Apoptosis of T47D cells and HC-toxin induced Apoptosis might not be mediated through direct increase in Caspase-3/7 activity. We have identified potent activities of antiproliferation, Apoptosis, and cell cycle arrest of trichostatin A and HC-toxin in Estrogen Receptor positive human breast Cancer cell line T47D.