2. Academic Validation
  3. Determination of beta2-agonists by ion chromatography with direct conductivity detection

Determination of beta2-agonists by ion chromatography with direct conductivity detection

  • J Pharm Biomed Anal. 2005 Jun 1;38(1):166-72. doi: 10.1016/j.jpba.2004.11.060.
Suhui Shen 1,  Jin Ouyang,  Willy R G Baeyens,  Joris R Delanghe,  Yiping Yang
Affiliations

Affiliation

  • 1 Department of Chemistry, No. 44, Beijing Normal University, Beijing 100875, PR China.
PMID: 15907636 DOI: 10.1016/j.jpba.2004.11.060
Abstract

A simple method for the simultaneous detection of four beta2-agonists (salbutamol, fenoterol, clorprenaline, and clenbuterol) using ion chromatography (IC) with direct conductivity detection (CD) based on their ionization in acidic medium without chemical suppression is presented. The mixture of 1.8 mM HNO3 and 2% (v/v) acetonitrile was used as eluent. The method could be applied to the determination of the beta2-agonists in pharmaceutical preparations. The recovery of salbutamol and clenbuterol in tablets was more than 97% (n=3) and the relative standard deviation (n=11) less than 2.8%. With the proposed method, salbutamol could also be successfully detected in human plasma. In a single chromatographic run, the four beta2-agonists can be separated and determined in less than 8 min. The linear ranges were of 7.0-1.4 x 10(3)ng/ml for salbutamol, 34-7.8 x 10(3)ng/ml for fenoterol, 8.0-1.6 x 10(3)ng/ml for clorprenaline, and 25-7.5 x 10(3)ng/ml for clenbuterol. The detection limits were 2.0 ng/ml for salbutamol, 10 ng/ml for fenoterol, 3.0 ng/ml for clorprenaline, and 10 ng/ml for clenbuterol.

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