2. Academic Validation
  3. Multidrug resistance-associated protein 9 (ABCC12) is present in mouse and boar sperm

Multidrug resistance-associated protein 9 (ABCC12) is present in mouse and boar sperm

  • Biochem J. 2007 Aug 15;406(1):31-40. doi: 10.1042/BJ20070292.
Nobuhito Ono 1,  Ingrid Van der Heijden,  George L Scheffer,  Koen Van de Wetering,  Elizabeth Van Deemter,  Marcel De Haas,  Arjan Boerke,  Bart M Gadella,  Dirk G De Rooij,  Jacques J Neefjes,  Tom A M Groothuis,  Lauran Oomen,  Lenny Brocks,  Toshihisa Ishikawa,  Piet Borst
Affiliations

Affiliation

  • 1 Division of Molecular Biology and Center of Biomedical Genetics, The Netherlands Cancer Institute, Plesmanlaan 121, 1066 CX Amsterdam, The Netherlands.
PMID: 17472575 DOI: 10.1042/BJ20070292
Abstract

The human and murine genes for MRP9 (multidrug resistance-associated protein 9; ABCC12) yield many alternatively spliced RNAs. Using a panel of monoclonal Antibodies, we detected full-length Mrp9 only in testicular germ cells and mouse sperm; we obtained no evidence for the existence of the truncated 100 kDa MRP9 protein reported previously. In contrast with other MRPs, neither murine Mrp9 nor the human MRP9 produced in MRP9-transfected HEK-293 cells (human embryonic kidney cells) appears to contain N-linked carbohydrates. In mouse and boar sperm, Mrp9 localizes to the midpiece, a structure containing all sperm mitochondria. However, immunolocalization microscopy and cell fractionation studies with transfected HEK-293 cells and mouse testis show that MRP9/Mrp9 does not localize to mitochondria. In HEK-293 cells, it is predominantly localized in the endoplasmic reticulum. We have been unable to demonstrate transport by MRP9 of substrates transported by other MRPs, such as drug conjugates and other organic anions.

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