2. Academic Validation
  3. TOPORS functions as a SUMO-1 E3 ligase for chromatin-modifying proteins

TOPORS functions as a SUMO-1 E3 ligase for chromatin-modifying proteins

  • J Proteome Res. 2007 Oct;6(10):3918-23. doi: 10.1021/pr0703674.
Pooja Pungaliya 1,  Diptee Kulkarni,  Hye-Jin Park,  Henderson Marshall,  Haiyan Zheng,  Henry Lackland,  Ahamed Saleem,  Eric H Rubin
Affiliations

Affiliation

  • 1 Department of Pharmacology, The Cancer Institute of New Jersey, Robert Wood Johnson Medical School, University of Medicine and Dentistry of New Jersey, 195 Little Albany Street, New Brunswick, New Jersey 08901, USA.
PMID: 17803295 DOI: 10.1021/pr0703674
Abstract

TOPORS is the first example of a protein with both ubiquitin and SUMO-1 E3 ligase activity and has been implicated as a tumor suppressor in several different malignancies. To gain insight into the cellular role of TOPORS, a proteomic screen was performed to identify candidate sumoylation substrates. The results indicate that many of the putative substrates are involved in chromatin modification or transcriptional regulation. Transfection studies confirmed mammalian Sin3A as a sumoylation substrate for TOPORS. These findings suggest that TOPORS may function as a tumor suppressor by regulating mSin3A and other proteins involved in chromatin modification.

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