2. Academic Validation
  3. Comprehensive study of alkaloids from Crinum asiaticum var. sinicum assisted by HPLC-DAD-SPE-NMR

Comprehensive study of alkaloids from Crinum asiaticum var. sinicum assisted by HPLC-DAD-SPE-NMR

  • J Nat Prod. 2011 Mar 25;74(3):411-9. doi: 10.1021/np100819n.
Chien-Kuang Chen 1,  Feng-Hsu Lin,  Li-Hong Tseng,  Chia-Ling Jiang,  Shoei-Sheng Lee
Affiliations

Affiliation

  • 1 School of Pharmacy, College of Medicine, National Taiwan University, 1, Sec. 1, Jen-Ai Rd, Taipei 10051, Taiwan, Republic of China.
PMID: 21314165 DOI: 10.1021/np100819n
Abstract

A comprehensive study of the Alkaloids presentin the leaves of Crinum asiaticum var. sinicum, assisted by HPLC-SPE-NMR, led to the characterization of 21 compounds of similar polarity on an analytical scale. Thirteen of these were isolated for further structural confirmation. Seven are proved to be new, namely, (+)-siculine (4), 1-epijosephinine (11), 7-methoxycrinamabine (10), 2-O-acetylcrinamabine (16), 3-O-acetyl-8-O-demethylmaritidine (17), 2-O-acetylbulbisine (18), and 1-O-acetylbulbisine (19). In addition, dihydrovittatine (6) and 8-O-demethyloxomaritidine (21) were isolated for the first time from Nature, although they have been prepared previously as synthetic products. Their structures were established by spectroscopic analysis.

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