2. Academic Validation
  3. Development of on-line high performance liquid chromatography (HPLC)-biochemical detection methods as tools in the identification of bioactives

Development of on-line high performance liquid chromatography (HPLC)-biochemical detection methods as tools in the identification of bioactives

  • Int J Mol Sci. 2012;13(3):3101-3133. doi: 10.3390/ijms13033101.
Christiaan J Malherbe 1,  Dalene De Beer 1,  Elizabeth Joubert 1 2
Affiliations

Affiliations

  • 1 Post-Harvest and Wine Technology Division, Agricultural Research Council, Infruitec-Nietvoorbij, Private Bag X5026, Stellenbosch, 7599, South Africa.
  • 2 Department of Food Science, Stellenbosch University, Private Bag X1, Matieland, Stellenbosch, 7602, South Africa.
PMID: 22489144 DOI: 10.3390/ijms13033101
Abstract

Biochemical detection (BCD) methods are commonly used to screen plant extracts for specific biological activities in batch assays. Traditionally, bioactives in the most active extracts were identified through time-consuming bio-assay guided fractionation until single active compounds could be isolated. Not only are isolation procedures often tedious, but they could also lead to artifact formation. On-line coupling of BCD assays to high performance liquid chromatography (HPLC) is gaining ground as a high resolution screening technique to overcome problems associated with pre-isolation by measuring the effects of compounds post-column directly after separation. To date, several on-line HPLC-BCD assays, applied to whole plant extracts and mixtures, have been published. In this review the focus will fall on enzyme-based, receptor-based and antioxidant assays.

Keywords

HPLC; antioxidant; bioactive phytochemicals; biochemical detection; enzyme inhibition; receptor affinity.

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