2. Academic Validation
  3. Development of a fluorescent chalcone library and its application in the discovery of a mouse embryonic stem cell probe

Development of a fluorescent chalcone library and its application in the discovery of a mouse embryonic stem cell probe

  • Chem Commun (Camb). 2012 Jul 7;48(53):6681-3. doi: 10.1039/c2cc31662e.
Sung-Chan Lee 1,  Nam-Young Kang,  Sung-Jin Park,  Seong-Wook Yun,  Yogeswari Chandran,  Young-Tae Chang
Affiliations

Affiliation

  • 1 Laboratory of Bioimaging Probe Development, Singapore Bioimaging Consortium, Agency for Science, Technology and Research, Biopolis, Singapore 138667.
PMID: 22641464 DOI: 10.1039/c2cc31662e
Abstract

We report the first fluorescent diamino-chalcone library and its application in the discovery of a mouse embryonic stem cell (mESC) probe. CDg4, a novel green fluorescent mESC probe was discovered through a high-content image based screening of 160 members of the chalcone library. Interestingly, the molecular binding target of CDg4 was identified as the glycogen of the stem cell colony surface, rather than a conventional protein target from an intracellular source.

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