2. Academic Validation
  3. BMP-2, VEGF and bFGF synergistically promote the osteogenic differentiation of rat bone marrow-derived mesenchymal stem cells

BMP-2, VEGF and bFGF synergistically promote the osteogenic differentiation of rat bone marrow-derived mesenchymal stem cells

  • Biotechnol Lett. 2013 Mar;35(3):301-8. doi: 10.1007/s10529-012-1084-3.
Yan Bai 1,  Peipei Li,  Guangfu Yin,  Zhongbing Huang,  Xiaoming Liao,  Xianchun Chen,  Yadong Yao
Affiliations

Affiliation

  • 1 College of Materials Science and Engineering, Sichuan University, Chengdu, 610064, People's Republic of China.
PMID: 23143174 DOI: 10.1007/s10529-012-1084-3
Abstract

Mesenchymal stem cells (MSCs) were treated with bone morphogenetic protein-2 (BMP-2), vascular endothelial growth factor (VEGF) and basic Fibroblast Growth Factor (bFGF) dose-dependently and time-dependently. Together they caused a strong synergistic effect on the osteogenic differentiation of MSCs, with lower concentrations of each factor being enough to show the synergistic promotion (50 ng BMP-2/ml, 1 ng VEGF/ml and 10 ng bFGF/ml). When both VEGF and bFGF were added in the early proliferating stage (the first 7 days) and BMP-2 was added in the late differentiation stage (the last 7 days), osteogenic differentiation of MSCs could be enhanced more effectively.

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