2. Academic Validation
  3. Reversible off-on fluorescence probe for hypoxia and imaging of hypoxia-normoxia cycles in live cells

Reversible off-on fluorescence probe for hypoxia and imaging of hypoxia-normoxia cycles in live cells

  • J Am Chem Soc. 2012 Dec 5;134(48):19588-91. doi: 10.1021/ja310049d.
Shodai Takahashi 1,  Wen Piao,  Yuriko Matsumura,  Toru Komatsu,  Tasuku Ueno,  Takuya Terai,  Toshiaki Kamachi,  Masahiro Kohno,  Tetsuo Nagano,  Kenjiro Hanaoka
Affiliations

Affiliation

  • 1 Faculty of Pharmaceutical Sciences, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
PMID: 23157219 DOI: 10.1021/ja310049d
Abstract

We report a fully reversible off-on fluorescence probe for hypoxia. The design employs QSY-21 as a Förster resonance energy transfer (FRET) acceptor and cyanine dye Cy5 as a FRET donor, based on our finding that QSY-21 undergoes one-electron bioreduction to the radical under hypoxia, with an absorbance decrease at 660 nm. At that point, FRET can no longer occur, and the dye becomes strongly fluorescent. Upon recovery of normoxia, the radical is immediately reoxidized to QSY-21, with loss of fluorescence due to restoration of FRET. We show that this probe, RHyCy5, can monitor repeated hypoxia-normoxia cycles in live cells.

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