2. Academic Validation
  3. Deficiency in SLC25A1, encoding the mitochondrial citrate carrier, causes combined D-2- and L-2-hydroxyglutaric aciduria

Deficiency in SLC25A1, encoding the mitochondrial citrate carrier, causes combined D-2- and L-2-hydroxyglutaric aciduria

  • Am J Hum Genet. 2013 Apr 4;92(4):627-31. doi: 10.1016/j.ajhg.2013.03.009.
Benjamin Nota 1,  Eduard A Struys,  Ana Pop,  Erwin E Jansen,  Matilde R Fernandez Ojeda,  Warsha A Kanhai,  Martijn Kranendijk,  Silvy J M van Dooren,  Marianna R Bevova,  Erik A Sistermans,  Aggie W M Nieuwint,  Magalie Barth,  Tawfeg Ben-Omran,  Georg F Hoffmann,  Pascale de Lonlay,  Marie T McDonald,  Alf Meberg,  Ania C Muntau,  Jean-Marc Nuoffer,  Rossella Parini,  Marie-Hélène Read,  Axel Renneberg,  René Santer,  Thomas Strahleck,  Emile van Schaftingen,  Marjo S van der Knaap,  Cornelis Jakobs,  Gajja S Salomons
Affiliations

Affiliation

  • 1 Metabolic Unit, Department of Clinical Chemistry, Neuroscience Campus Amsterdam, VU University Medical Center, 1081 HV Amsterdam, the Netherlands. [email protected]
PMID: 23561848 DOI: 10.1016/j.ajhg.2013.03.009
Abstract

The Krebs cycle is of fundamental importance for the generation of the energetic and molecular needs of both prokaryotic and eukaryotic cells. Both enantiomers of metabolite 2-hydroxyglutarate are directly linked to this pivotal biochemical pathway and are found elevated not only in several cancers, but also in different variants of the neurometabolic disease 2-hydroxyglutaric aciduria. Recently we showed that cancer-associated IDH2 germline mutations cause one variant of 2-hydroxyglutaric aciduria. Complementary to these findings, we now report recessive mutations in SLC25A1, the mitochondrial citrate carrier, in 12 out of 12 individuals with combined D-2- and L-2-hydroxyglutaric aciduria. Impaired mitochondrial citrate efflux, demonstrated by stable isotope labeling experiments and the absence of SLC25A1 in fibroblasts harboring certain mutations, suggest that SLC25A1 deficiency is pathogenic. Our results identify defects in SLC25A1 as a cause of combined D-2- and L-2-hydroxyglutaric aciduria.

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