2. Academic Validation
  3. Tylvalosin exhibits anti-inflammatory property and attenuates acute lung injury in different models possibly through suppression of NF-κB activation

Tylvalosin exhibits anti-inflammatory property and attenuates acute lung injury in different models possibly through suppression of NF-κB activation

  • Biochem Pharmacol. 2014 Jul 1;90(1):73-87. doi: 10.1016/j.bcp.2014.04.015.
Zhanzhong Zhao 1,  Xiangfang Tang 2,  Xinghui Zhao 3,  Minhong Zhang 4,  Weijian Zhang 5,  Shaohua Hou 6,  Weifeng Yuan 7,  Hongfu Zhang 8,  Lijun Shi 9,  Hong Jia 10,  Lin Liang 11,  Zhi Lai 12,  Junfeng Gao 13,  Keyu Zhang 14,  Ling Fu 15,  Wei Chen 16
Affiliations

Affiliations

  • 1 State Key Laboratory of Animal Nutrition, Department of Veterinary Medicine, Beijing Institute of Animal Husbandry and Veterinary Medicine, Chinese Academy of Agricultural Sciences, No. 2 Yuanmingyuan West Road, Haidian District, Beijing 100193, People's Republic of China; Beijing Institute of Biotechnology, No. 20 Dongdajie Street, Fengtai District, Beijing 100071, People's Republic of China. Electronic address: [email protected].
  • 2 State Key Laboratory of Animal Nutrition, Department of Veterinary Medicine, Beijing Institute of Animal Husbandry and Veterinary Medicine, Chinese Academy of Agricultural Sciences, No. 2 Yuanmingyuan West Road, Haidian District, Beijing 100193, People's Republic of China. Electronic address: [email protected].
  • 3 Beijing Institute of Biotechnology, No. 20 Dongdajie Street, Fengtai District, Beijing 100071, People's Republic of China. Electronic address: [email protected].
  • 4 State Key Laboratory of Animal Nutrition, Department of Veterinary Medicine, Beijing Institute of Animal Husbandry and Veterinary Medicine, Chinese Academy of Agricultural Sciences, No. 2 Yuanmingyuan West Road, Haidian District, Beijing 100193, People's Republic of China. Electronic address: [email protected].
  • 5 Shanghai Municipal Animal Innocuous Treatment Center, No. 50 Lane 4088, Puwei Road, Fengxian District, Shanghai 201415, People's Republic of China. Electronic address: [email protected].
  • 6 State Key Laboratory of Animal Nutrition, Department of Veterinary Medicine, Beijing Institute of Animal Husbandry and Veterinary Medicine, Chinese Academy of Agricultural Sciences, No. 2 Yuanmingyuan West Road, Haidian District, Beijing 100193, People's Republic of China. Electronic address: [email protected].
  • 7 State Key Laboratory of Animal Nutrition, Department of Veterinary Medicine, Beijing Institute of Animal Husbandry and Veterinary Medicine, Chinese Academy of Agricultural Sciences, No. 2 Yuanmingyuan West Road, Haidian District, Beijing 100193, People's Republic of China. Electronic address: [email protected].
  • 8 State Key Laboratory of Animal Nutrition, Department of Veterinary Medicine, Beijing Institute of Animal Husbandry and Veterinary Medicine, Chinese Academy of Agricultural Sciences, No. 2 Yuanmingyuan West Road, Haidian District, Beijing 100193, People's Republic of China. Electronic address: [email protected].
  • 9 State Key Laboratory of Animal Nutrition, Department of Veterinary Medicine, Beijing Institute of Animal Husbandry and Veterinary Medicine, Chinese Academy of Agricultural Sciences, No. 2 Yuanmingyuan West Road, Haidian District, Beijing 100193, People's Republic of China. Electronic address: [email protected].
  • 10 State Key Laboratory of Animal Nutrition, Department of Veterinary Medicine, Beijing Institute of Animal Husbandry and Veterinary Medicine, Chinese Academy of Agricultural Sciences, No. 2 Yuanmingyuan West Road, Haidian District, Beijing 100193, People's Republic of China. Electronic address: [email protected].
  • 11 State Key Laboratory of Animal Nutrition, Department of Veterinary Medicine, Beijing Institute of Animal Husbandry and Veterinary Medicine, Chinese Academy of Agricultural Sciences, No. 2 Yuanmingyuan West Road, Haidian District, Beijing 100193, People's Republic of China. Electronic address: [email protected].
  • 12 Biopharmavet Institute, No.161 Zhenye Road, Songjiang District, Shanghai 201619, People's Republic of China. Electronic address: [email protected].
  • 13 Biopharmavet Institute, No.161 Zhenye Road, Songjiang District, Shanghai 201619, People's Republic of China. Electronic address: [email protected].
  • 14 Key Laboratory for Veterinary Drug Safety Evaluation and Residue Research, Department of Pharmacy, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, No. 518 Ziyue Road, Minhang District, Shanghai 200241, People's Republic of China. Electronic address: [email protected].
  • 15 Beijing Institute of Biotechnology, No. 20 Dongdajie Street, Fengtai District, Beijing 100071, People's Republic of China. Electronic address: [email protected].
  • 16 Beijing Institute of Biotechnology, No. 20 Dongdajie Street, Fengtai District, Beijing 100071, People's Republic of China. Electronic address: [email protected].
PMID: 24792436 DOI: 10.1016/j.bcp.2014.04.015
Abstract

Tylvalosin, a new broad-spectrum, third-generation macrolides, may exert a variety of pharmacological activities. Here, we report on its anti-oxidative and anti-inflammatory activity in RAW 264.7 macrophages and mouse treated with lipopolysaccharide (LPS) as well as piglet challenged with porcine reproductive and respiratory syndrome virus (PRRSV). Tylvalosin treatment markedly decreased IL-8, IL-6, IL-1β, PGE2, TNF-α and NO levels in vitro and in vivo. LPS and PRRSV-induced Reactive Oxygen Species (ROS) production, and the lipid peroxidation in mice lung tissues reduced after tylvalosin treatments. In mouse acute lung injury model induced by LPS, tylvalosin administration significantly attenuated tissues injury, and reduced the inflammatory cells recruitment and activation. The evaluated Phospholipase A2 (PLA2) activity and the increased expressions of cPLA2-IVA, p-cPLA2-IVA and sPLA2-IVE were lowered by tylvalosin. Consistent with the mouse results, tylvalosin pretreatment attenuated piglet lung scores with improved growth performance and normal rectal temperature in piglet model induced by PRRSV. Furthermore, tylvalosin attenuated the IκBα phosphorylation and degradation, and blocked the NF-κB p65 translocation. These results indicate that in addition to its direct antimicrobial effect, tylvalosin exhibits anti-inflammatory property and attenuates acute lung injury through suppression of NF-κB activation.

Keywords

Acute lung injury; Anti-inflammation; NF-κB; Tylvalosin.

Figures
Products