2. Academic Validation
  3. Comprehensive identification of active triterpenoid metabolites in frankincense using a coupling strategy

Comprehensive identification of active triterpenoid metabolites in frankincense using a coupling strategy

  • J Chromatogr B Analyt Technol Biomed Life Sci. 2014 Jul 15:963:90-8. doi: 10.1016/j.jchromb.2014.05.054.
Yuanyan Liu 1,  Zhenli Liu 2,  Cheng Lu 3,  Jianbei Li 4,  Zhangchi Ning 5,  Zhiqian Song 6,  Chun Wang 6,  Zhiyong Du 6,  Xinmiao Lu 7,  Siyu Zhao 7,  Aiping Lu 8
Affiliations

Affiliations

  • 1 School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing, China. Electronic address: [email protected].
  • 2 Institution of Basic Theory, China Academy of Chinese Medical Sciences, Beijing, China. Electronic address: [email protected].
  • 3 Institute of Basic Research in Clinical Medicine, China Academy of Chinese Medical Sciences, Beijing, China.
  • 4 Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.
  • 5 School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing, China; Institution of Basic Theory, China Academy of Chinese Medical Sciences, Beijing, China.
  • 6 Institution of Basic Theory, China Academy of Chinese Medical Sciences, Beijing, China.
  • 7 School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing, China.
  • 8 Institute of Basic Research in Clinical Medicine, China Academy of Chinese Medical Sciences, Beijing, China; Institute for Advancing Translational Medicine in Bone & Joint Diseases, School of Chinese Medicine, Hong Kong Baptist University, Kowloon Tong, Hong Kong SAR, China. Electronic address: [email protected].
PMID: 24946158 DOI: 10.1016/j.jchromb.2014.05.054
Abstract

Frankincense resins are extensively used as natural remedies in regions ranging from North Africa to China. Triterpenoid metabolites from frankincense exhibit notable anti-inflammatory and anti-tumor properties. In the present paper, without the use of an isolation process, the fragmentation rules and NMR spectral characteristics of triterpenoid metabolites in frankincense are summarized through a coupling method using high performance liquid chromatography-diode array detection/electrospray ionization tandem mass spectrometry (HPLC-DAD/ESI-MS(n)) combined with HPLC-nuclear magnetic resonance (NMR) experiments. Based on this groundwork, a coupling strategy for the comprehensive metabolic profiling of active triterpenoid metabolites from enriched fractions of frankincense was developed. The proposed strategy may serve as a method for the holistic screening of bioactive metabolites in complex TCM samples.

Keywords

Frankincense resins; Mass spectrometry; Metabolic profiling; Nuclear magnetic resonance; Triterpenoid metabolites.

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