2. Academic Validation
  3. c-Jun transcriptionally regulates alpha 1, 2-fucosyltransferase 1 (FUT1) in ovarian cancer

c-Jun transcriptionally regulates alpha 1, 2-fucosyltransferase 1 (FUT1) in ovarian cancer

  • Biochimie. 2014 Dec;107 Pt B:286-92. doi: 10.1016/j.biochi.2014.09.015.
Na Gao 1,  Juanjuan Liu 2,  Dawo Liu 2,  Yingying Hao 2,  Limei Yan 2,  Yanan Ma 3,  Huiyu Zhuang 2,  Zhenhua Hu 2,  Jian Gao 2,  Zhihai Yang 4,  Hong Shi 4,  Bei Lin 5
Affiliations

Affiliations

  • 1 Department of Obstetrics and Gynecology, Shengjing Hospital Affiliated to China Medical University, Shenyang 110004, China; Department of Obstetrics and Gynecology, First Affiliated Hospital of Dalian Medical University, Dalian 116011, China.
  • 2 Department of Obstetrics and Gynecology, Shengjing Hospital Affiliated to China Medical University, Shenyang 110004, China.
  • 3 Department of Biostatistics and Epidemiology, School of Public Health, China Medical University, Shenyang 110001, China.
  • 4 Department of Obstetrics and Gynecology, First Affiliated Hospital of Dalian Medical University, Dalian 116011, China.
  • 5 Department of Obstetrics and Gynecology, Shengjing Hospital Affiliated to China Medical University, Shenyang 110004, China. Electronic address: [email protected].
PMID: 25239830 DOI: 10.1016/j.biochi.2014.09.015
Abstract

Alpha 1, 2-fucosyltransferase (FUT 1/2) is a rate-limiting Enzyme that catalyzes the synthesis of Lewis y, a cell membrane-associated carbohydrate antigen. In human ovarian Cancer, the upregulated expression of FUT1 and Lewis y is associated with advanced pathological stages and involved in cell proliferation, migration and invasion. However, the mechanism underlying the upregulation of FUT1 is largely unknown. Here, we identify an AP-1 binding site in FUT1 promoter in ovarian Cancer cells. c-Jun promotes FUT1 expression, thereby enhancing Lewis y biosynthesis in various ovarian Cancer cell lines. Moreover, EMSA, luciferase activity and ChIP assays demonstrate c-Jun directly interacts with FUT1 promoter. Furthermore, FUT1 mediates c-Jun-induced cell proliferation in ovarian Cancer cells. In human ovarian Cancer samples, c-Jun overexpression is linked to malignant degree and positively correlated to FUT1 and Lewis y expression. Taken together, c-Jun could transcriptionally modulate FUT1 expression in ovarian Cancer, implicating the potential application of c-Jun inhibitors for human ovarian Cancer therapy.

Keywords

FUT1; Lewis y; Ovarian cancer; Transcriptional regulation; c-Jun.

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