2. Academic Validation
  3. RILP interacts with HOPS complex via VPS41 subunit to regulate endocytic trafficking

RILP interacts with HOPS complex via VPS41 subunit to regulate endocytic trafficking

  • Sci Rep. 2014 Dec 2;4:7282. doi: 10.1038/srep07282.
Xiaosi Lin 1,  Ting Yang 1,  Shicong Wang 1,  Zhen Wang 1,  Ye Yun 1,  Lixiang Sun 1,  Yunhe Zhou 1,  Xiaohui Xu 1,  Chihiro Akazawa 2,  Wanjin Hong 3,  Tuanlao Wang 1
Affiliations

Affiliations

  • 1 School of Pharmaceutical Sciences, State Key Laboratory of Cellular Stress Biology, Xiamen University, Xiamen, Fujian, China, 361005.
  • 2 Department of Biophysics and Biochemistry, Graduate School of Health Sciences, Tokyo Medical and Dental University, Yushima 1-5-45, Bunkyo-Ku, Tokyo 113-8519, Japan.
  • 3 1] School of Pharmaceutical Sciences, State Key Laboratory of Cellular Stress Biology, Xiamen University, Xiamen, Fujian, China, 361005 [2] Institute of Molecular and Cell Biology, A STAR (Agency for Science, Technology and Research), 61 Biopolis Drive, Singapore 138673, Singapore.
PMID: 25445562 DOI: 10.1038/srep07282
Abstract

The HOPS complex serves as a tethering complex with GEF activity for Ypt7p in yeast to regulate late endosomal membrane maturation. While the role of HOPS complex is well established in yeast cells, its functional and mechanistic aspects in mammalian cells are less well defined. In this study, we report that RILP, a downstream effector of Rab7, interacts with HOPS complex and recruits HOPS subunits to the late endosomal compartment. Structurally, the amino-terminal portion of RILP interacts with HOPS complex. Unexpectedly, this interaction is independent of Rab7. VPS41 subunit of HOPS complex was defined to be the major partner for interacting with RILP. The carboxyl-terminal region of VPS41 was mapped to be responsible for the interaction. Functionally, either depletion of VPS41 by shRNA or overexpression of VPS41 C-terminal half retarded EGF-induced degradation of EGFR. These results suggest that interaction of RILP with HOPS complex via VPS41 plays a role in endocytic trafficking of EGFR.

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