2. Academic Validation
  3. Targeted disruption of PDE3B, but not PDE3A, protects murine heart from ischemia/reperfusion injury

Targeted disruption of PDE3B, but not PDE3A, protects murine heart from ischemia/reperfusion injury

  • Proc Natl Acad Sci U S A. 2015 Apr 28;112(17):E2253-62. doi: 10.1073/pnas.1416230112.
Youn Wook Chung 1,  Claudia Lagranha 2,  Yong Chen 3,  Junhui Sun 2,  Guang Tong 4,  Steven C Hockman 5,  Faiyaz Ahmad 5,  Shervin G Esfahani 6,  Dahae H Bae 6,  Nazari Polidovitch 7,  Jian Wu 7,  Dong Keun Rhee 5,  Beom Seob Lee 8,  Marjan Gucek 3,  Mathew P Daniels 6,  Christine A Brantner 6,  Peter H Backx 9,  Elizabeth Murphy 2,  Vincent C Manganiello 10
Affiliations

Affiliations

  • 1 Cardiovascular and Pulmonary Branch, Yonsei Cardiovascular Research Institute, Yonsei University College of Medicine, Seoul 120-752, Korea; [email protected] [email protected].
  • 2 Systems Biology Center.
  • 3 Proteomics Core Facility, and.
  • 4 Systems Biology Center, Department of Cardiovascular Surgery, Guangzhou General Hospital of Guangzhou Military Command, Guangzhou, Guangdong 510010, China;
  • 5 Cardiovascular and Pulmonary Branch.
  • 6 Electron Microscopy Core Facility, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892;
  • 7 Departments of Physiology and Medicine, University of Toronto, Toronto, ON M5S 3A8, Canada;
  • 8 Yonsei Cardiovascular Research Institute, Yonsei University College of Medicine, Seoul 120-752, Korea; Graduate Program in Science for Aging, Yonsei University, Seoul 120-752, Korea; and.
  • 9 Departments of Physiology and Medicine, University of Toronto, Toronto, ON M5S 3A8, Canada; Division of Cardiology, University Health Network, Toronto, ON M5S 3E2, Canada.
  • 10 Cardiovascular and Pulmonary Branch, [email protected] [email protected].
PMID: 25877153 DOI: 10.1073/pnas.1416230112
Abstract

Although inhibition of cyclic nucleotide phosphodiesterase type 3 (PDE3) has been reported to protect rodent heart against ischemia/reperfusion (I/R) injury, neither the specific PDE3 isoform involved nor the underlying mechanisms have been identified. Targeted disruption of PDE3 subfamily B (PDE3B), but not of PDE3 subfamily A (PDE3A), protected mouse heart from I/R injury in vivo and in vitro, with reduced infarct size and improved cardiac function. The cardioprotective effect in PDE3B(-/-) heart was reversed by blocking cAMP-dependent PKA and by paxilline, an inhibitor of mitochondrial calcium-activated K channels, the opening of which is potentiated by cAMP/PKA signaling. Compared with WT mitochondria, PDE3B(-/-) mitochondria were enriched in antiapoptotic Bcl-2, produced less Reactive Oxygen Species, and more frequently contacted transverse tubules where PDE3B was localized with caveolin-3. Moreover, a PDE3B(-/-) mitochondrial fraction containing connexin-43 and caveolin-3 was more resistant to CA(2+)-induced opening of the mitochondrial permeability transition pore. Proteomics analyses indicated that PDE3B(-/-) heart mitochondria fractions were enriched in buoyant ischemia-induced caveolin-3-enriched fractions (ICEFs) containing cardioprotective proteins. Accumulation of proteins into ICEFs was PKA dependent and was achieved by ischemic preconditioning or treatment of WT heart with the PDE3 Inhibitor cilostamide. Taken together, these findings indicate that PDE3B deletion confers cardioprotective effects because of cAMP/PKA-induced preconditioning, which is associated with the accumulation of proteins with cardioprotective function in ICEFs. To our knowledge, our study is the first to define a role for PDE3B in cardioprotection against I/R injury and suggests PDE3B as a target for cardiovascular therapies.

Keywords

PDE3B−/− mice; ischemia/reperfusion injury; membrane repair; protein kinase A; signalosome.

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