2. Academic Validation
  3. Antioxidant homoisoflavonoids from Polygonatum odoratum

Antioxidant homoisoflavonoids from Polygonatum odoratum

  • Food Chem. 2015 Nov 1:186:63-8. doi: 10.1016/j.foodchem.2015.02.058.
Xiaoling Zhou 1,  Zhang Yuping 2,  Huading Zhao 2,  Junsheng Liang 1,  Yi Zhang 1,  Shuyun Shi 3
Affiliations

Affiliations

  • 1 Hunan Academy of Forest Sciences, Changsha 410004, PR China; Key Laboratory of Hunan Tree Clones Breeding, Changsha 410004, PR China.
  • 2 College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, PR China.
  • 3 College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, PR China. Electronic address: [email protected].
PMID: 25976792 DOI: 10.1016/j.foodchem.2015.02.058
Abstract

Polygonatum odoratum is widely used as a traditional food supplement and herbal medicine with strong antioxidant activity. However, systematic investigation of its Antioxidants was limited. Ethanol extract of P. odoratum was fractioned on macroporous absorptive resin (D101) column. A bioassay-guided purification of flavonoid-rich fraction (IC50 value at 74.1 ± 11.9 μg/mL for DPPH scavenging) was realised via high-speed counter-current chromatography (HSCCC) using petroleum ether-ethyl acetate-methanol-water (2:3:3:2, v/v/v/v) as the solvent system combination with Sephadex LH-20 column chromatography (CC) eluting with MeCN-MeOH (1:1, v/v). Three novel homoisoflavonoids (1-3), along with eight homoisoflavonoids (4-11), were isolated. Their structures were elucidated by interpretating various spectroscopic data. All the isolated homoisoflavonoids showed potent antioxidant activities, while compounds 1, 4, and 6 with dihydroxylated B-rings exhibited stronger antioxidant activities (IC50 values at 3.8 ± 0.5, 4.9 ± 0.3 and 3.9 ± 0.4 μg/mL) than ascorbic acid (IC50 value at 5.3 ± 0.6 μg/mL).

Keywords

Antioxidant; Homoisoflavonoid; Polygonatum odoratum; Structural elucidation.

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