2. Academic Validation
  3. Cyclin-dependent kinase 11(p110) (CDK11(p110)) is crucial for human breast cancer cell proliferation and growth

Cyclin-dependent kinase 11(p110) (CDK11(p110)) is crucial for human breast cancer cell proliferation and growth

  • Sci Rep. 2015 May 20:5:10433. doi: 10.1038/srep10433.
Yubing Zhou 1,  Chao Han 1,  Duolu Li 1,  Zujiang Yu 2,  Fengmei Li 3,  Feng Li 1,  Qi An 1,  Huili Bai 4,  Xiaojian Zhang 1,  Zhenfeng Duan 1 5,  Quancheng Kan 1
Affiliations

Affiliations

  • 1 Department of Pharmacy, The First Affiliated Hospital of Zhengzhou University, 1 Jianshe East Road, Zhengzhou 450052, China.
  • 2 Department of Infectious Diseases, The First Affiliated Hospital of Zhengzhou University, 1 Jianshe East Road, Zhengzhou 450052, China.
  • 3 Department of Obstetrics and Gynecology, Zhengzhou Central Hospital Affiliated to Zhengzhou University, 195 Tongbai Road, Zhengzhou 450007, China.
  • 4 Department of Pathology, Zhengzhou Central Hospital Affiliated to Zhengzhou University, 195 Tongbai Road, Zhengzhou 450007, China.
  • 5 Sarcoma Molecular Biology Laboratory, Center for Sarcoma and Connective Tissue Oncology, Massachusetts General Hospital and Harvard Medical School, 55 Fruit Street, Boston, MA, USA.
PMID: 25990212 DOI: 10.1038/srep10433
Abstract

Cyclin-dependent kinases (CDKs) play important roles in the development of many types of cancers by binding with their paired cyclins. However, the function of CDK11 larger protein isomer, CDK11(p110), in the tumorigenesis of human breast Cancer remains unclear. In the present study, we explored the effects and molecular mechanisms of CDK11(p110) in the proliferation and growth of breast Cancer cells by determining the expression of CDK11(p110) in breast tumor tissues and examining the phenotypic changes of breast Cancer cells after CDK11(p110) knockdown. We found that CDK11(p110) was highly expressed in breast tumor tissues and cell lines. Tissue microarray analysis showed that elevated CDK11(p110) expression in breast Cancer tissues significantly correlated with poor differentiation, and was also associated with advanced TNM stage and poor clinical prognosis for breast Cancer patients. In vitro knockdown of CDK11(p110) by siRNA significantly inhibited cell growth and migration, and dramatically induced Apoptosis in breast Cancer cells. Flow cytometry demonstrated that cells were markedly arrested in G1 phase of the cell cycle after CDK11(p110) downregulation. These findings suggest that CDK11(p110) is critical for the proliferation and growth of breast Cancer cells, which highlights CDK11(p110) may be a promising therapeutic target for the treatment of breast Cancer.

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