2. Academic Validation
  3. The Protective Effect of Resveratrol on Concanavalin-A-Induced Acute Hepatic Injury in Mice

The Protective Effect of Resveratrol on Concanavalin-A-Induced Acute Hepatic Injury in Mice

  • Gastroenterol Res Pract. 2015:2015:506390. doi: 10.1155/2015/506390.
Yingqun Zhou 1,  Kan Chen 1,  Lei He 1,  Yujing Xia 1,  Weiqi Dai 1,  Fan Wang 1,  Jingjing Li 1,  Sainan Li 1,  Tong Liu 1,  Yuanyuan Zheng 1,  Jianrong Wang 2,  Wenxia Lu 2,  Qin Yin 3,  Yuqing Zhou 3,  Jie Lu 1,  Hongfei Teng 4,  Chuanyong Guo 1
Affiliations

Affiliations

  • 1 Department of Gastroenterology, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai 200072, China.
  • 2 Department of Gastroenterology, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai 200072, China ; The First Clinical Medical College of Nanjing Medical University, Nanjing 210029, China.
  • 3 Department of Gastroenterology, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai 200072, China ; The First Affiliated Hospital of Soochow University, Suzhou 215006, China.
  • 4 Department of Gastroenterology, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai 200072, China ; Department of General Surgery, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai 200072, China.
PMID: 26089871 DOI: 10.1155/2015/506390
Abstract

Pharmacologic Relevance. Resveratrol, an antioxidant derived from grapes, has been reported to modulate the inflammatory process. In this study, we investigated the effects of resveratrol and its mechanism of protection on concanavalin-A- (ConA-) induced liver injury in mice. Materials and Methods. Acute autoimmune hepatitis was induced by ConA (20 mg/kg) in Balb/C mice; mice were treated with resveratrol (10, 20, and 30 mg/kg) daily by oral gavage for fourteen days prior to a single intravenous injection of ConA. Eight hours after injection, histologic grading, proinflammatory cytokine levels, and Hedgehog pathway activity were determined. Results. After ConA injection, the cytokines IL-2, IL-6, and TNF-α were increased, and Sonic Hedgehog (Shh), Glioblastoma- (Gli-) 1, and Patched (Ptc) levels significantly increased. Pretreatment with resveratrol ameliorated the pathologic effects of ConA-induced autoimmune hepatitis and significantly inhibited IL-2, IL-6, TNF-α, Shh, Gli-1, and Ptc. The effects of resveratrol on the Hedgehog pathway were studied by western blotting and immunohistochemistry. Resveratrol decreased Shh expression, possibly by inhibiting Shh expression in order to reduce Gli-1 and Ptc expression. Conclusion. Resveratrol protects against ConA-induced autoimmune hepatitis by decreasing cytokines expression in mice. The decreases seen in Gli-1 and Ptc may correlate with the amelioration of Hedgehog pathway activity.

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