Concanavalin A (agarose)
Based on 1 Customer Validation
Concanavalin A (agarose) is composed of Concanavalin A (HY-P2149) conjugated with agarose. Concanavalin A (ConA) is a selective competitive binder targeting the specific carbohydrate structures of glucose and mannose, inducing mitosis, and exhibiting certain cytotoxicity, hepatotoxicity, and teratogenicity. Concanav in A (agarose) can be used for in vivo blood glucose monitoring in diabetes, and for "fishing out" specific glycoproteins or removing sugar impurities from complex samples.
For research use only. We do not sell to patients.
- CAS No.: 11028-71-0
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Concanavalin A (agarose) is primarily used for protein purification from complex samples, such as cytokines, membrane proteins, and serum glycoproteins; or for removing unwanted glycoprotein impurities from samples; or for studying the interaction strength between glycoproteins and ConA (e.g., under different elution conditions)[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:Mouse spleen cells, human B lymphocytes, human T lymphocytes, murine T lymphocytes
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Concentration:3-6 μg/mL (optimal), 10 μg/mL, 25 μg/mL
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Incubation Time:72 h
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Result:Concanavalin A (agarose) directly stimulated B cells and T cells to synthesize DNA and proliferate, with the optimal concentration ranging from 3 to 6 μg/mL. The dose-response curve declined sharply when the concentration exceeded 10 μg/mL. It induced the production of interferon in spleen cells from C57BL/6 mice at 25 μg/mL. The mitogenic effect on lymphocytes was age-dependent, with impaired responses observed in lymphocytes from older individuals and murine T lymphocyte subsets.
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Animal Model:Rabbits (body weight 2 kg, 2.9 kg, 3.1 kg, 1.3 kg)[1]
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Dosage:Rabbits: 1 mg, 2.4 mg, 4 mg, 7 mg, 15 mg
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Administration:intravenous injection
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Result:Caused no symptoms in rabbits.
Administration of 7 mg ConA to a 1.3 kg rabbit resulted in death within 2 days, while 15 mg ConA injected into a 2 kg rabbit induced intravascular erythrocyte agglutination and the rabbit was euthanized.
Chemical Information
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CAS No. 11028-71-0
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Appearance Liquid (Density: 1.434±0.06 g/cm3)
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Color Colorless to light yellow
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SMILES
[Concanavalin A (agarose)]
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
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Data Sheet (268 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
[1]. Ballerstadt R, et al. Concanavalin A for in vivo glucose sensing: a biotoxicity review. Biosens Bioelectron. 2006 Aug 15;22(2):275-84. [Content Brief]
[2]. Li W, et, al. Concanavalin A: a potential anti-neoplastic agent targeting apoptosis, autophagy and anti-angiogenesis for cancer therapeutics. Biochem Biophys Res Commun. 2011 Oct 22;414(2):282-6. [Content Brief]
[3]. Cantelli A, et al. Concanavalin A-Rose Bengal bioconjugate for targeted Gram-negative antimicrobial photodynamic therapy. J Photochem Photobiol B. 2020 Mar 13;206:111852. [Content Brief]
[4]. Zhou Y, et al. The Protective Effect of Resveratrol on Concanavalin-A-Induced Acute Hepatic Injury in Mice. Gastroenterol Res Pract. 2015;2015:506390. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Concanavalin A (agarose) Related Classifications
HY-P2149A Related Classifications
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