Concanavalin A (agarose)
Based on 1 Customer Validation
Concanavalin A (agarose) is composed of Concanavalin A (HY-P2149) conjugated with agarose. Concanavalin A (ConA) is a selective competitive binder targeting the specific carbohydrate structures of glucose and mannose, inducing mitosis, and exhibiting certain cytotoxicity, hepatotoxicity, and teratogenicity. Concanav in A (agarose) can be used for in vivo blood glucose monitoring in diabetes, and for "fishing out" specific glycoproteins or removing sugar impurities from complex samples.
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- CAS No.: 11028-71-0
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Stockage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Activité biologique
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Chemical Information
- Protocole
- Pureté et documentation
- Références
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Help & FAQs
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HY-P2149
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Activité biologique
Description
In Vitro
Concanavalin A (agarose) is primarily used for protein purification from complex samples, such as cytokines, membrane proteins, and serum glycoproteins; or for removing unwanted glycoprotein impurities from samples; or for studying the interaction strength between glycoproteins and ConA (e.g., under different elution conditions)[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:Mouse spleen cells, human B lymphocytes, human T lymphocytes, murine T lymphocytes
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Concentration:3-6 μg/mL (optimal), 10 μg/mL, 25 μg/mL
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Incubation Time:72 h
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Result:Concanavalin A (agarose) directly stimulated B cells and T cells to synthesize DNA and proliferate, with the optimal concentration ranging from 3 to 6 μg/mL. The dose-response curve declined sharply when the concentration exceeded 10 μg/mL. It induced the production of interferon in spleen cells from C57BL/6 mice at 25 μg/mL. The mitogenic effect on lymphocytes was age-dependent, with impaired responses observed in lymphocytes from older individuals and murine T lymphocyte subsets.
In Vivo
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Animal Model:Rabbits (body weight 2 kg, 2.9 kg, 3.1 kg, 1.3 kg)[1]
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Dosage:Rabbits: 1 mg, 2.4 mg, 4 mg, 7 mg, 15 mg
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Administration:intravenous injection
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Result:Caused no symptoms in rabbits.
Administration of 7 mg ConA to a 1.3 kg rabbit resulted in death within 2 days, while 15 mg ConA injected into a 2 kg rabbit induced intravascular erythrocyte agglutination and the rabbit was euthanized.
Chemical Information
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CAS No. 11028-71-0
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Appearance Liquid (Density: 1.434±0.06 g/cm3)
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Color Colorless to light yellow
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SMILES
[Concanavalin A (agarose)]
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
Please store the product under the recommended conditions in the Certificate of Analysis.
Pureté et documentation
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Fiche technique (270 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Instruction de manipulation (2659 KB)
Références
[1]. Ballerstadt R, et al. Concanavalin A for in vivo glucose sensing: a biotoxicity review. Biosens Bioelectron. 2006 Aug 15;22(2):275-84. [Content Brief]
[2]. Li W, et, al. Concanavalin A: a potential anti-neoplastic agent targeting apoptosis, autophagy and anti-angiogenesis for cancer therapeutics. Biochem Biophys Res Commun. 2011 Oct 22;414(2):282-6. [Content Brief]
[3]. Cantelli A, et al. Concanavalin A-Rose Bengal bioconjugate for targeted Gram-negative antimicrobial photodynamic therapy. J Photochem Photobiol B. 2020 Mar 13;206:111852. [Content Brief]
[4]. Zhou Y, et al. The Protective Effect of Resveratrol on Concanavalin-A-Induced Acute Hepatic Injury in Mice. Gastroenterol Res Pract. 2015;2015:506390. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Concanavalin A (agarose) Related Classifications
HY-P2149A Related Classifications
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