2. Academic Validation
  3. Surface immobilization of a protease through an inhibitor-derived affinity ligand: a bioactive surface with defensive properties against an inhibitor

Surface immobilization of a protease through an inhibitor-derived affinity ligand: a bioactive surface with defensive properties against an inhibitor

  • Chem Commun (Camb). 2015 Sep 28;51(75):14263-6. doi: 10.1039/c5cc05652g.
Zengchao Tang 1,  Yafei Luan,  Dan Li,  Hui Du,  David M Haddleton,  Hong Chen
Affiliations

Affiliation

  • 1 College of Chemistry, Chemical Engineering and Materials Science, Soochow University, 199 Ren'ai Rd, Suzhou 215123, Jiangsu, P. R. China. [email protected] [email protected].
PMID: 26264862 DOI: 10.1039/c5cc05652g
Abstract

The concept of enzyme immobilization via an inhibitor-derived peptide was developed. This method of immobilization was shown to be advantageous over physical adsorption and covalent bonding in retaining the enzymatic activity. Moreover, the surface-immobilized enzyme exhibited resistance against its inhibitor due to the occupation of an inhibitor binding site on the enzyme.

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