2. Academic Validation
  3. Silibinin derivatives as anti-prostate cancer agents: Synthesis and cell-based evaluations

Silibinin derivatives as anti-prostate cancer agents: Synthesis and cell-based evaluations

  • Eur J Med Chem. 2016 Feb 15:109:36-46. doi: 10.1016/j.ejmech.2015.12.041.
Bao Vue 1,  Sheng Zhang 1,  Xiaojie Zhang 1,  Konstantinos Parisis 1,  Qiang Zhang 2,  Shilong Zheng 2,  Guangdi Wang 3,  Qiao-Hong Chen 4
Affiliations

Affiliations

  • 1 Department of Chemistry, California State University, Fresno, 2555 E. San Ramon Avenue, M/S SB70, Fresno, CA 93740, USA.
  • 2 RCMI Cancer Research Center Xavier University of Louisiana, 1 Drexel Drive, New Orleans, LA 70125, USA.
  • 3 RCMI Cancer Research Center Xavier University of Louisiana, 1 Drexel Drive, New Orleans, LA 70125, USA; Department of Chemistry, Xavier University of Louisiana, 1 Drexel Drive, New Orleans, LA 70125, USA.
  • 4 Department of Chemistry, California State University, Fresno, 2555 E. San Ramon Avenue, M/S SB70, Fresno, CA 93740, USA. Electronic address: [email protected].
PMID: 26748997 DOI: 10.1016/j.ejmech.2015.12.041
Abstract

This study aims to systematically explore the alkylation effect of 7-OH in silibinin and 2,3-dehydrosilibinin on the antiproliferative potency toward three prostate Cancer cell lines. Eight 7-O-alkylsilibinins, eight 7-O-alkyl-2,3-dehydrosilibinins, and eight 3,7-O-dialkyl-2,3-dehydrosilibinins have been synthesized from commercially available silibinin for the in vitro cell-based evaluation. The WST-1 cell proliferation assay indicates that nineteen out of twenty-four silibinin derivatives have significantly improved antiproliferative potency when compared with silibinin. 7-O-Methylsilibinin (2) and 7-O-ethylsilibinin (3) have been identified as the most potent compounds with 98- and 123-fold enhanced potency against LNCaP human androgen-dependent prostate Cancer cell line. Among 2,3-dehydrosilibinin derivatives, 7-O-methyl-2,3-dehydrosilibinin (10) and 7-O-ethyl-2,3-dehydrosilibinin (11) have been identified as the optimal compounds with the highest potency towards both androgen-dependent LNCaP and androgen-independent PC-3 prostate Cancer cell lines. 7-O-Ethyl-2,3-dehydrosilibinin (11) was demonstrated to arrest PC-3 cell cycle at the G0/G1 phase and to induce PC-3 cell Apoptosis. The findings in this study suggest that antiproliferative potency of silibinin and 2,3-dehydrosilibinin can be appreciably enhanced through suitable chemical modifications on the phenolic hydroxyl group at C-7 and that introduction of a chemical moiety with the potential to improve bioavailability through a linker to 7-OH in silibinin and 2,3-dehydrosilibinin would be a feasible strategy for the development of silibinin derivatives as anti-prostate Cancer agents.

Keywords

Anti-proliferative activity; Cell apoptosis; Prostate cancer; Silibinin derivatives; Structure–activity relationship.

Figures