2. Academic Validation
  3. THBS1 is induced by TGFB1 in the cancer stroma and promotes invasion of oral squamous cell carcinoma

THBS1 is induced by TGFB1 in the cancer stroma and promotes invasion of oral squamous cell carcinoma

  • J Oral Pathol Med. 2016 Nov;45(10):730-739. doi: 10.1111/jop.12430.
Samir Kumar Pal 1 2,  Chi Thi Kim Nguyen 1,  Kei-Ichi Morita 3,  Yoshio Miki 2 4,  Kou Kayamori 1,  Akira Yamaguchi 1 2 5,  Kei Sakamoto 6
Affiliations

Affiliations

  • 1 Department of Oral Pathology, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, Tokyo, Japan.
  • 2 Global Center of Excellence (GCOE) Program, International Research Center for Molecular Science in Tooth and Bone Diseases, Tokyo Medical and Dental University, Tokyo, Japan.
  • 3 Department of Oral Surgery, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, Tokyo, Japan.
  • 4 Department of Molecular Genetics, Medical Research Institute, Tokyo Medical and Dental University, Tokyo, Japan.
  • 5 Oral Health Science Center, Tokyo Dental College, Tokyo, Japan.
  • 6 Department of Oral Pathology, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, Tokyo, Japan. [email protected].
PMID: 26850833 DOI: 10.1111/jop.12430
Abstract

Background: THBS1 (thrombospondin-1) is the extracellular matrix (ECM) protein that affects diverse cellular activities. It constitutes the tumor stroma, but the role of THBS1 in oral squamous cell carcinoma (OSCC) development is unclear. The aim of this study was to clarify the relevance of THBS1 in the pathogenesis of OSCC.

Materials and methods: The expression of THBS1 was examined in 44 OSCC by immunohistochemical analysis and in 43 OSCC by cDNA microarray analysis. Cell Culture experiments were conducted using human OSCC cell lines HSC3 and HO1N1 and mouse fibroblast ST2 cells to examine the effect of TGFB1 on THBS1 expression, and the effect of THBS1 on cellular behaviors.

Results: THBS1 was specifically induced in the tumor microenvironment of OSCC. THBS1 appeared to be produced mainly by the stromal cells, but also by OSCC cells. TGFB1 stimulated THBS1 expression in ST2, primary fibroblasts, and the OSCC cells. THBS1 promoted migration and invasion of HSC3 and HO1N1 in transwell migration assays. THBS1 stimulated the expression of MMP3 (matrix metalloprotease 3), MMP9, MMP11, and MMP13 in ST2 cells and MMP3, MMP11, and MMP13 in HO1N1 cells. The RGD peptide suppressed the THBS1-stimulated migration and upregulation of MMP11 and MMP13.

Conclusions: THBS1 is a tumor-specific ECM protein that is induced by TGFB1 and promotes migration of Cancer cells and stimulates the expression of MMPs partly through the Integrin signaling, thereby favoring OSCC invasion.

Keywords

TGFB1; THBS1; TSP-1; integrin; oral cancer; thrombospondin.

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