2. Academic Validation
  3. α-Phellandrene, a cyclic monoterpene, attenuates inflammatory response through neutrophil migration inhibition and mast cell degranulation

α-Phellandrene, a cyclic monoterpene, attenuates inflammatory response through neutrophil migration inhibition and mast cell degranulation

  • Life Sci. 2016 Sep 1;160:27-33. doi: 10.1016/j.lfs.2016.07.008.
Halmisson D'Arley S Siqueira 1,  Benedito S Neto 1,  Damião P Sousa 2,  Bruno S Gomes 1,  Francilene Vieira da Silva 1,  Francisco V M Cunha 3,  Carlos W S Wanderley 2,  Gabriel Pinheiro 2,  André G F Cândido 2,  Deysi V T Wong 2,  Ronaldo A Ribeiro 2,  Roberto C P Lima-Júnior 2,  Francisco A Oliveira 1
Affiliations

Affiliations

  • 1 Medicinal Plants Research Center, Federal University of Piauí, (unnumbered), 64049-550 Teresina, Piauí, Brazil; Physiology and Pharmacology Department, Medicine Faculty, Federal University of Ceará, Cel. Nunes de Melo 1127, 60430-270 Fortaleza, CE, Brazil.
  • 2 Pharmaceutical Sciences Department, Federal University of Paraíba, Jardim Universitário, 58051-970 João Pessoa, PB, Brazil; Physiology and Pharmacology Department, Medicine Faculty, Federal University of Ceará, Cel. Nunes de Melo 1127, 60430-270 Fortaleza, CE, Brazil.
  • 3 Medicinal Plants Research Center, Federal University of Piauí, (unnumbered), 64049-550 Teresina, Piauí, Brazil; Physiology and Pharmacology Department, Medicine Faculty, Federal University of Ceará, Cel. Nunes de Melo 1127, 60430-270 Fortaleza, CE, Brazil. Electronic address: [email protected].
PMID: 27449945 DOI: 10.1016/j.lfs.2016.07.008
Abstract

Aims: We aimed to investigate the modulating effect of α-phellandrene on neutrophil migration and mast cell degranulation processes.

Main methods: Male Wistar rats or Swiss mice were treated p.o. with vehicle (3% Tween 80, p.o.), α-phellandrene (50, 100, or 200mg/kg, p.o.), or dexamethasone (0.5mg/kg, p.o.) 1h before carrageenan injection. Then, the neutrophil migration in 6-day-old air pouches or peritoneal cavities. The leukocyte rolling and adhesion were measured in real time and assessed by intravital microscopy. ELISA was used to detect TNF-α and IL-6 in peritoneal lavage. Compound 48/80-induced mast cell degranulation was assessed in mesenteric rat tissues.

Key findings: In all the tested doses, α-phellandrene prevented carrageenan-induced neutrophil accumulation (P<0.05). As detected by intravital microscopy, α-phellandrene also inhibited leukocyte rolling and adhesion, as well as significantly inhibited the production of the pro-inflammatory cytokines TNF-α and IL-6. Moreover, the degranulation of compound 48/80-induced mast cells was also inhibited by α-phellandrene (P<0.001).

Significance: These results suggest that α-phellandrene plays an important role as an anti-inflammatory agent through neutrophil migration modulation and mast cell stabilization.

Keywords

Air-pouch; Inflammation; Mast cell; Neutrophil; Peritonitis; α-Phellandrene.

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