2. Academic Validation
  3. Tiron ameliorates oxidative stress and inflammation in a murine model of airway remodeling

Tiron ameliorates oxidative stress and inflammation in a murine model of airway remodeling

  • Int Immunopharmacol. 2016 Oct;39:172-180. doi: 10.1016/j.intimp.2016.07.025.
Nagla A El-Sherbeeny 1,  Zeinab A Hassan 2,  Hayam Ateyya 3
Affiliations

Affiliations

  • 1 Department of Clinical Pharmacology, Faculty of Medicine, Suez canal University, Egypt. Electronic address: [email protected].
  • 2 Department of Histology and Cell Biology, Faculty of Medicine, Zagazig University, Egypt.
  • 3 Department of Clinical Pharmacology, Faculty of Medicine, Cairo University, Egypt.
PMID: 27485290 DOI: 10.1016/j.intimp.2016.07.025
Abstract

Airway remodeling includes lung structural changes that have a role in the irreversibility of pulmonary dysfunction shown in chronic bronchial asthmatics. The current experiment investigated the effect of the mitochondrial antioxidant, tiron in comparison with dexamethasone (DEXA) on airway remodeling in chronic asthma. Sensitized BALB/c mice were challenged with ovalbumin (OVA) aerosol for 8weeks, OVA sensitized-challenged mice were treated with either DEXA or tiron, respectively. After that, lung tissue and bronchoaveolar lavage fluid (BALF) were used for measurement of different biological markers. Lungs were examined for histopathological changes and immunohistochemistry. Upon comparing with vehicle treated Animals, trion or DEXA treatment significantly reduced eosinophils, lymphocytes, neutrophils and macrophages count in the BALF. Both drugs significantly alleviated chronic OVA-induced oxidative stress as illustrated by decreased pulmonary malondialdenhyde (MDA) and increased glutathione (GSH) and superoxide dismutase (SOD) levels. Asthmatic mice exhibited elevated levels of NOx, IL-13 and TGF-β1 that were reduced by DEXA and tiron. Histopathological changes and increased immunoreactivity of nuclear factor-Kappa B (NF-κ B) in OVA-challenged mice were minimized by tiron and DEXA treatment. In conclusion, in this model of chronic asthma DEXA and tiron ameliorated airway remodeling and inflammation in experimental chronic asthma with no difference between the effect of tiron and DEXA. Tiron has a potential role as adjuvant treatment in chronic asthma.

Keywords

Airway remodeling; Antioxidants; Dexamethasone; TGF-β1; Tiron.

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