2. Academic Validation
  3. Isolation and chatacterization of homoisoflavonoids from Dracaena cochinchinensis and their osteogenic activities in mouse mesenchymal stem cells

Isolation and chatacterization of homoisoflavonoids from Dracaena cochinchinensis and their osteogenic activities in mouse mesenchymal stem cells

  • J Pharm Biomed Anal. 2016 Sep 10;129:466-472. doi: 10.1016/j.jpba.2016.07.017.
Xing Xu 1,  Kejun Cheng 2,  Wenliang Cheng 2,  Tianxing Zhou 2,  Min Jiang 3,  Jinbiao Xu 4
Affiliations

Affiliations

  • 1 Chemical Biology Center, Lishui Institute of Agricultural Sciences, 827 Liyang Stress, Lishui, Zhejiang 323000, China; Shanghai Key Laboratory for Bone and Joint Diseases, Shanghai Institute of Traumatology and Orthopaedics, Shanghai Ruijin Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200025, China.
  • 2 Chemical Biology Center, Lishui Institute of Agricultural Sciences, 827 Liyang Stress, Lishui, Zhejiang 323000, China.
  • 3 Shanghai Key Laboratory for Bone and Joint Diseases, Shanghai Institute of Traumatology and Orthopaedics, Shanghai Ruijin Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200025, China. Electronic address: [email protected].
  • 4 Chemical Biology Center, Lishui Institute of Agricultural Sciences, 827 Liyang Stress, Lishui, Zhejiang 323000, China; University of Science and Technology of China, Hefei, Anhui 230026, China. Electronic address: [email protected].
PMID: 27497307 DOI: 10.1016/j.jpba.2016.07.017
Abstract

Two new homoisoflavonoids, dracaeconolide A (1) and dracaeconolide B (2), together with ten known compounds, namely (3R)-7,4'-dihydroxy-8-methoxyhomoisoflavane (3), (3R)-7-hydroxy-3-(4-hydroxybenzyl)chromane (4), (3R)-7,4'-dihydroxy-5-methoxy-homoisoflavane (5), (3R)-6,4'-dihydroxy-8-methoxyhomoisoflavan (6), 7,4'-dihydroxy-8-methylflavan (7), (2R)-7,4'-dihydroxy-5-methoxy-8-methylflavan (8), 5,4'-dihydroxy-7-methoxy-6-methylflavane (9), 7,4'-dihydroxy-3'-methoxyflavan (10), 7,4'-dihydroxyflavan (11), 4,4'-dihydroxy-2,6-dimethoxydihydrochlcone (12), were isolated from the red resin of Dracaena cochinchinensis (dragon's blood, DB). All the compounds were then evaluated for their effects on mouse bone marrow-derived mesenchymal stem cells (MSCs) proliferation using CCK8 assay and their abilities in promoting MSCs differentiating into osteoblast through the assay of Alkaline Phosphatase (ALP) activity in vitro. Compounds 2, 3, 4, 7, 9, and 11, at a concentration of 10μM with no cytotoxicity, significantly promoted MSC osteogenic differentiation by increasing the levels of ALP activity to percents of 159.6±5.9, 167.6±10.9, 162.0±1.4, 151.3±4.0, 171.0±8.2, and 169.9±7.3 in relative to the control, respectively. The results of ALP staining were in accordance to that of ALP activity.

Keywords

Alkaline phosphatase; Dracaena cochinchinensis; Homoisoflavonoids; Mesenchymal stem cells; Osteogenesis; Osteoporosis.

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