2. Academic Validation
  3. Effects of Sanguis Draconis on Perforator Flap Survival in Rats

Effects of Sanguis Draconis on Perforator Flap Survival in Rats

  • Molecules. 2016 Sep 26;21(10):1262. doi: 10.3390/molecules21101262.
Yang Zhang 1,  Xiaobing Cai 2,  Lifeng Shen 3,  Xiaowen Huang 4,  Xuping Wang 5,  Yinan Lan 6,  Dan Shou 7
Affiliations

Affiliations

  • 1 Department of Medicine, Zhejiang Academy of Traditional Chinese Medicine, Hangzhou 310007, Zhejiang, China. [email protected].
  • 2 Department of Orthopedic Surgery, Lishui Central Hospital, Lishui 323000, Zhejiang, China. [email protected].
  • 3 Department of Orthopedic Surgery, Zhejiang Provincial Tongde Hospital, Hangzhou 310012, Zhejiang, China. [email protected].
  • 4 Department of Medicine, Zhejiang Academy of Traditional Chinese Medicine, Hangzhou 310007, Zhejiang, China. [email protected].
  • 5 Department of Medicine, Zhejiang Academy of Traditional Chinese Medicine, Hangzhou 310007, Zhejiang, China. [email protected].
  • 6 Department of Orthopedic Surgery, Lishui Central Hospital, Lishui 323000, Zhejiang, China. [email protected].
  • 7 Department of Medicine, Zhejiang Academy of Traditional Chinese Medicine, Hangzhou 310007, Zhejiang, China. [email protected].
PMID: 27681718 DOI: 10.3390/molecules21101262
Abstract

Sanguis draconis, a resin known to improve blood circulation, relieve pain, stimulate tissue regeneration, and heal wounds, is widely used in clinical practice. In this study, we prepared an ethanol extract of sanguis draconis (EESD) containing 75.08 mg/g of dracorhodin. The experiment was carried out on 20 rats that were divided into two groups, a control group (n = 10) and an EESD group (n = 10). All the rats underwent a perforator FLAP surgery, after which post-operative abdominal compressions of EESD were given to the EESD group for seven days, while the control group received saline. FLAP survival percentages were determined after seven days, and were found to be significantly higher in the EESD group than in the control group. Results of laser Doppler flowmetry (LDF) showed that perforator flaps in the EESD group had higher perfusion values than those of the control group. The FLAP tissues were stained with hematoxylin and eosin, followed by immunohistochemical evaluation. Superoxide dismutase (SOD) expression and micro-vessel development markedly increased in the EESD group, while malondialdehyde (MDA) levels decreased. This is the first study to investigate the effect of sanguis draconis on perforator FLAP survival. Our results demonstrate that sanguis draconis can improve perforator FLAP survival in rats by promoting microvessel regeneration and blood perfusion.

Keywords

dracorhodin; flap survival; microvessels; perforator flaps; perfusion; sanguis draconis.

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