2. Academic Validation
  3. Production of N-acyl homoserine lactones by the sponge-associated marine actinobacteria Salinispora arenicola and Salinispora pacifica

Production of N-acyl homoserine lactones by the sponge-associated marine actinobacteria Salinispora arenicola and Salinispora pacifica

  • FEMS Microbiol Lett. 2017 Jan 1;364(2). doi: 10.1093/femsle/fnx002.
Utpal Bose 1,  Catharine A Ortori 2,  Sarir Sarmad 2,  David A Barrett 2,  Amitha K Hewavitharana 1,  Mark P Hodson 1 3,  John A Fuerst 4,  P Nicholas Shaw 1,  Rich Boden
Affiliations

Affiliations

  • 1 School of Pharmacy, The University of Queensland, Brisbane, QLD 4072, Australia.
  • 2 Centre for Analytical Bioscience, School of Pharmacy, University of Nottingham, University Park, Nottingham NG7 2RD, UK.
  • 3 Metabolomics Australia, Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, Brisbane, QLD 4072, Australia.
  • 4 School of Chemistry and Molecular Biosciences, The University of Queensland, Brisbane, QLD 4072, Australia.
PMID: 28087611 DOI: 10.1093/femsle/fnx002
Abstract

The structures of acyl homoserine lactone (AHL) compounds and their quantification were accomplished using an integrated liquid chromatography-mass spectrometry approach. The precursor and product ions, along with retention times of peaks, were searched against an in-house database of AHLs and structures confirmed by accurate mass and by comparison with authentic AHL standards. The two compounds, N-(3-oxodecanoyl)-L-homoserine lactone and N-(3-oxododecanoyl)-L-homoserine lactone, were characterised and quantified in Salinispora sp. cultures.

Keywords

homoserine lactone; liquid chromatography; marine bacteria; mass spectrometry.

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