2. Academic Validation
  3. Hierarchical assembly of centriole subdistal appendages via centrosome binding proteins CCDC120 and CCDC68

Hierarchical assembly of centriole subdistal appendages via centrosome binding proteins CCDC120 and CCDC68

  • Nat Commun. 2017 Apr 19;8:15057. doi: 10.1038/ncomms15057.
Ning Huang 1 2,  Yuqing Xia 1 2,  Donghui Zhang 1 2,  Song Wang 1 2,  Yitian Bao 1 2,  Runsheng He 1 2,  Junlin Teng 1 2,  Jianguo Chen 1 2 3
Affiliations

Affiliations

  • 1 Key Laboratory of Cell Proliferation and Differentiation of the Ministry of Education, College of Life Sciences, Peking University, Beijing 100871, China.
  • 2 State Key Laboratory of Membrane Biology, College of Life Sciences, Peking University, Beijing 100871, China.
  • 3 Center for Quantitative Biology, Peking University, Beijing 100871, China.
PMID: 28422092 DOI: 10.1038/ncomms15057
Abstract

In animal cells, the centrosome is the main microtubule-organizing centre where microtubules are nucleated and anchored. The centriole subdistal appendages (SDAs) are the key structures that anchor microtubules in interphase cells, but the composition and assembly mechanisms of SDAs are not well understood. Here, we reveal that centrosome-binding proteins, coiled-coil domain containing (CCDC) 120 and CCDC68 are two novel SDA components required for hierarchical SDA assembly in human cells. CCDC120 is anchored to SDAs by ODF2 and recruits CEP170 and Ninein to the centrosome through different coiled-coil domains at its N terminus. CCDC68 is a CEP170-interacting protein that competes with CCDC120 in recruiting CEP170 to SDAs. Furthermore, CCDC120 and CCDC68 are required for centrosome microtubule anchoring. Our findings elucidate the molecular basis for centriole SDA hierarchical assembly and microtubule anchoring in human interphase cells.

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