2. Academic Validation
  3. Ultrasound-assisted lipase catalyzed hydrolysis of aspirin methyl ester

Ultrasound-assisted lipase catalyzed hydrolysis of aspirin methyl ester

  • Ultrason Sonochem. 2018 Jan;40(Pt A):587-593. doi: 10.1016/j.ultsonch.2017.08.004.
Pranali P Chiplunkar 1,  Xiaoman Zhao 2,  Prerana D Tomke 3,  Jennifer Noro 4,  Bo Xu 5,  Qiang Wang 5,  Carla Silva 4,  Amit P Pratap 6,  Artur Cavaco-Paulo 7
Affiliations

Affiliations

  • 1 International Joint Research Laboratory for Textile and Fiber Bioprocesses, Jiangnan University, Wuxi 214122, China; Department of Oils, Oleochemicals and Surfactants Technology, Institute of Chemical Technology, Matunga (E), Mumbai 400019, India.
  • 2 International Joint Research Laboratory for Textile and Fiber Bioprocesses, Jiangnan University, Wuxi 214122, China; Jiangsu Engineering Technology Research Center for Functional Textiles, Institute of Chemical Technology, Matunga (E), Mumbai 400019, India; Key Laboratory of Science and Technology of Eco-Textiles, Ministry of Education, Jiangnan University, Wuxi 214122, Jiangsu, China.
  • 3 International Joint Research Laboratory for Textile and Fiber Bioprocesses, Jiangnan University, Wuxi 214122, China; Department of Chemical Engineering, Institute of Chemical Technology, Matunga (E), Mumbai 400019, India.
  • 4 Centre of Biological Engineering, University of Minho, Campus de Gualtar, 4710-057, Braga, Portugal.
  • 5 International Joint Research Laboratory for Textile and Fiber Bioprocesses, Jiangnan University, Wuxi 214122, China.
  • 6 Department of Oils, Oleochemicals and Surfactants Technology, Institute of Chemical Technology, Matunga (E), Mumbai 400019, India.
  • 7 International Joint Research Laboratory for Textile and Fiber Bioprocesses, Jiangnan University, Wuxi 214122, China; Centre of Biological Engineering, University of Minho, Campus de Gualtar, 4710-057, Braga, Portugal. Electronic address: [email protected].
PMID: 28946463 DOI: 10.1016/j.ultsonch.2017.08.004
Abstract

The ultrasound-assisted hydrolysis of aspirin methyl ester (AME) was investigated using immobilized Candida antarctica Lipase B (CALB) (1%) in the presence of solvents like triolein, chloroform (CHCl3) and dichloromethane (DCM). The effect of ultrasound and the role of water on the conversion rates have also been investigated. Proton nuclear magnetic resonance spectroscopic (1H NMR) was chosen to calculate hydrolysis convertion rates. We observed that lipase-ultrasound assisted hydrolysis of AME in the presence of triolein and water showed the highest hydrolysis conversion rate (65.3%). Herein low water amount played an important role as a nucleophile being crucial for the hydrolysis yields obtained. Lipase activity was affected by the conjugated action of ultrasound and solvents (35.75% of decrease), however not disturbing its hydrolytic efficiency. It was demonstrated that Lipase is able to hydrolyse AME to methyl 2-hydroxy benzoate (methyl salicylate), which applications include fragrance agents in food, beverages and cosmetics, or analgesic agent in liniments.

Keywords

Aspirin methyl ester; Hydrolysis; Lipase; Ultrasound; Water.

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