2. Academic Validation
  3. Identification and characterization of curcuminoids in turmeric using ultra-high performance liquid chromatography-quadrupole time of flight tandem mass spectrometry

Identification and characterization of curcuminoids in turmeric using ultra-high performance liquid chromatography-quadrupole time of flight tandem mass spectrometry

  • J Chromatogr A. 2017 Oct 27:1521:110-122. doi: 10.1016/j.chroma.2017.09.032.
Shuailong Jia 1,  Zhifeng Du 1,  Chengwu Song 2,  Shuna Jin 3,  Yang Zhang 4,  Yulin Feng 5,  Chaomei Xiong 1,  Hongliang Jiang 6
Affiliations

Affiliations

  • 1 Tongji School of Pharmacy, Huazhong University of Science and Technology, 13 Hangkong Road, Wuhan, Hubei, China.
  • 2 College of Pharmacy, Hubei University of Chinese Medicine, Wuhan, China.
  • 3 Key Laboratory of Environment and Health, Ministry of Education & Ministry of Environmental Protection, State Key Laboratory of Environmental Health (incubation), School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, China.
  • 4 Tongji Hospital, Huazhong University of Science and Technology, Wuhan, China.
  • 5 Jiangxi University of Traditional Chinese Medicine, Nanchang, China.
  • 6 Tongji School of Pharmacy, Huazhong University of Science and Technology, 13 Hangkong Road, Wuhan, Hubei, China. Electronic address: [email protected].
PMID: 28951052 DOI: 10.1016/j.chroma.2017.09.032
Abstract

A three-step strategy was developed for systematic characterization of curcuminoids in turmeric. Based on UHPLC-QTOF-MS/MS analysis, 89 curcuminoids including 16 novel ones were identified in the turmeric samples using this approach. During the identification process, false positive results were excluded by combining the positive and negative ESI-MS/MS analyses. Moreover, the characterization of the keto and enol forms of type A, B and C curcuminoids was first discussed and they were clearly distinguished using negative ESI-MS/MS method with UV spectra analyses. The structures of detected curcuminoids were identified and rationalized in both ion modes. Additionally, the fragmentation behaviors of the 15 types of curcuminoids were clearly illustrated in this work, which will be helpful for detection and identification of corresponding trace curcuminoids in complex turmeric samples using UHPLC-QTOF-MS/MS methods.

Keywords

Curcuminoids; Fragmentation patterns; Keto-enol tautomerism; Keywords; Turmeric; UHPLC-QTOF-MS/MS.

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