2. Academic Validation
  3. Kallikrein-related peptidase 14 is the second KLK protease targeted by the serpin vaspin

Kallikrein-related peptidase 14 is the second KLK protease targeted by the serpin vaspin

  • Biol Chem. 2018 Sep 25;399(9):1079-1084. doi: 10.1515/hsz-2018-0108.
David Ulbricht 1,  Catherine A Tindall 1,  Kathrin Oertwig 1,  Stefanie Hanke 2,  Norbert Sträter 2,  John T Heiker 1
Affiliations

Affiliations

  • 1 Institute of Biochemistry, Faculty of Life Sciences, Leipzig University, Brüderstrasse 34, D-04103 Leipzig, Germany.
  • 2 Institute of Bioanalytical Chemistry, Center for Biotechnology and Biomedicine, Leipzig University, D-04103 Leipzig, Germany.
PMID: 29494334 DOI: 10.1515/hsz-2018-0108
Abstract

Kallikrein-related peptidases KLK5, KLK7 and KLK14 are important proteases in skin desquamation and aberrant KLK activity is associated with inflammatory skin diseases such as Netherton syndrome but also with various serious forms of Cancer. Previously, we have identified KLK7 as the first protease target of Vaspin (Serpin A12). Here, we report KLK14 as a second KLK protease to be inhibited by Vaspin. In conclusion, Vaspin represents a multi-specific Serpin targeting the Kallikrein proteases KLK7 and KLK14, with distinct exosites regulating recognition of these target proteases and opposing effects of heparin binding on the inhibition reaction.

Keywords

adipokine; diabetes; exosite; inflammation; kallikrein; metabolic syndrome; obesity; serine protease; serpin A12; site-directed mutagenesis; structural biology.

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