2. Academic Validation
  3. Role of Membrane Cholesterol Levels in Activation of Lyn upon Cell Detachment

Role of Membrane Cholesterol Levels in Activation of Lyn upon Cell Detachment

  • Int J Mol Sci. 2018 Jun 19;19(6):1811. doi: 10.3390/ijms19061811.
Takao Morinaga 1 2,  Noritaka Yamaguchi 3,  Yuji Nakayama 4,  Masatoshi Tagawa 5 6,  Naoto Yamaguchi 7
Affiliations

Affiliations

  • 1 Division of Pathology and Cell Therapy, Chiba Cancer Center Research Institute, Chiba 260-8717, Japan. [email protected].
  • 2 Laboratory of Molecular Cell Biology, Graduate School of Pharmaceutical Sciences, Chiba University, Chiba 260-8675, Japan. [email protected].
  • 3 Laboratory of Molecular Cell Biology, Graduate School of Pharmaceutical Sciences, Chiba University, Chiba 260-8675, Japan. [email protected].
  • 4 Department of Biochemistry and Molecular Biology, Kyoto Pharmaceutical University, Kyoto 607-8414, Japan. [email protected].
  • 5 Division of Pathology and Cell Therapy, Chiba Cancer Center Research Institute, Chiba 260-8717, Japan. [email protected].
  • 6 Department of Molecular Biology and Oncology, Graduate School of Medicine, Chiba University, Chiba 260-8670, Japan. [email protected].
  • 7 Laboratory of Molecular Cell Biology, Graduate School of Pharmaceutical Sciences, Chiba University, Chiba 260-8675, Japan. [email protected].
PMID: 29921831 DOI: 10.3390/ijms19061811
Abstract

Cholesterol, a major component of the plasma membrane, determines the physicalproperties of biological membranes and plays a critical role in the assembly of membranemicrodomains. Enrichment or deprivation of membrane Cholesterol affects the activities of manysignaling molecules at the plasma membrane. Cell detachment changes the structure of the plasmamembrane and influences the localizations of lipids, including Cholesterol. Recent studies showedthat cell detachment changes the activities of a variety of signaling molecules. We previously reportedthat the localization and the function of the Src-family kinase Lyn are critically regulated by its membrane anchorage through lipid modifications. More recently, we found that the localization andthe activity of Lyn were changed upon cell detachment, although the manners of which vary betweencell types. In this review, we highlight the changes in the localization of Lyn and a role of cholesterolin the regulation of Lyn’s activation following cell detachment.

Keywords

Lyn activation; Src-family kinases; cell detachment; cell–scaffold interactions; cholesterol; membrane distribution; subcellular localization.

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