2. Academic Validation
  3. RFamide-related peptide-3 promotes alpha TC1 clone 6 cell survival likely via GPR147

RFamide-related peptide-3 promotes alpha TC1 clone 6 cell survival likely via GPR147

  • Peptides. 2018 Sep;107:39-44. doi: 10.1016/j.peptides.2018.07.009.
Wencheng Zhang 1,  Li Wang 1,  Xinwen Yu 1,  Aihua Jia 1,  Jie Ming 1,  Qiuhe Ji 2
Affiliations

Affiliations

  • 1 Department of Endocrinology, Xijing Hospital, Fourth Military Medical University, Xi'an, 169 Changle Road West, 710032, China.
  • 2 Department of Endocrinology, Xijing Hospital, Fourth Military Medical University, Xi'an, 169 Changle Road West, 710032, China. Electronic address: [email protected].
PMID: 30081043 DOI: 10.1016/j.peptides.2018.07.009
Abstract

Type 2 diabetes mellitus (T2DM) is closely related to islet alpha cell mass and viability. Several types of RFamide-related Peptides (RFRPs) are involved in regulating proliferation and function of islet cells. However, current understanding of the role of RFamide-related peptide-3 (RFRP-3) in pancreatic alpha cells is limited. Therefore, we investigated the expression of the RFRP-3 receptor, G protein-coupled receptor 147 (GPR147), in mouse islets and alpha TC1 clone 6 cells, and evaluated the function of RFRP-3 on alpha cells. We show that GPR147 is expressed in mouse islets and alpha cell lines. In addition, RFRP-3 promotes survival of alpha cells under conditions of hyperglycemia and serum starvation. Mechanistic evidence demonstrates that RFRP-3 activated PI3K/Akt and ERK1/2 signaling cascades and treatment with an antagonist of GPR147, 1-adamantanecarbonyl-Arg-Phe-NH₂ (RF9) blocked this function. These findings indicate a novel effect of RFRP-3 in promoting alpha cell survival, likely via GPR147.

Keywords

1-adamantanecarbonyl-Arg-Phe-NH₂(RF9); G protein-coupled receptor 147 (GPR147); Gonadotropin inhibitory hormone (GnIH); RFamide-related peptide-3 (RFRP-3).

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