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  3. Effect of Ribose Conformation on RNA Cleavage via Internal Transesterification

Effect of Ribose Conformation on RNA Cleavage via Internal Transesterification

  • J Am Chem Soc. 2018 Sep 26;140(38):11893-11897. doi: 10.1021/jacs.8b06313.
Fengmin Guo 1,  Zekun Yue 1,  Marko Trajkovski 2,  Xiaoping Zhou 1,  Dong Cao 1,  Qiang Li 1,  Baifan Wang 1,  Xin Wen 1,  Janez Plavec 2,  Qian Peng 1,  Zhen Xi 1,  Chuanzheng Zhou 1 3
Affiliations

Affiliations

  • 1 State Key Laboratory of Elemento-Organic Chemistry and Department of Chemical Biology, College of Chemistry , Nankai University , Tianjin 300071 , China.
  • 2 Slovenian NMR Centre, National Institute of Chemistry , University of Ljubljana, Faculty of Chemistry and Chemical Technology, Ljubljana, EN-FIST Centre of Excellence , Hajdrihova 19 , Ljubljana 1000 , Slovenia.
  • 3 Collaborative Innovation Center of Chemical Science and Engineering (Tianjin) , Tianjin 300071 , China.
PMID: 30207719 DOI: 10.1021/jacs.8b06313
Abstract

RNA cleavage via internal transesterification is a fundamental reaction involved in RNA processing and metabolism, and the regulation thereof. Herein, the influence of ribose conformation on this reaction was investigated with conformationally constrained ribonucleotides. RNA cleavage rates were found to decrease in the order South-constrained ribonucleotide > native ribonucleotide ≫ North-constrained counterpart, indicating that the ribose conformation plays an important role in modulating RNA cleavage via internal transesterification.

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