2. Academic Validation
  3. Elucidation of the Herbicidin Tailoring Pathway Offers Insights into Its Structural Diversity

Elucidation of the Herbicidin Tailoring Pathway Offers Insights into Its Structural Diversity

  • Org Lett. 2019 Mar 1;21(5):1374-1378. doi: 10.1021/acs.orglett.9b00066.
Hai-Xue Pan 1,  Zhang Chen,  Tianfang Zeng 2,  Wen-Bing Jin 1,  Yujie Geng,  Geng-Min Lin,  Juan Zhao 1,  Wei-Tao Li 1,  Zijun Xiong 3,  Sheng-Xiong Huang 3,  Xin Zhai 2,  Hung-Wen Liu,  Gong-Li Tang 1
Affiliations

Affiliations

  • 1 State Key Laboratory of Bio-organic and Natural Products Chemistry, Center for Excellence in Molecular Synthesis, Shanghai Institute of Organic Chemistry , University of Chinese Academy of Sciences (CAS), CAS, Shanghai 200032 , China.
  • 2 Key Laboratory of Structure-Based Drug Design & Discovery, Ministry of Education , Shenyang Pharmaceutical University , Shenyang 110016 , China.
  • 3 State Key Laboratory of Phytochemistry and Plant Resources in West China, Center for Excellence in Molecular Plant Sciences , Kunming Institute of Botany , CAS, Kunming 650201 , China.
PMID: 30763106 DOI: 10.1021/acs.orglett.9b00066
Abstract

The biosynthetic gene clusters for herbicidins ( hbc) and aureonuclemycin ( anm) were identified in Streptomyces sp. KIB-027 and Streptomyces aureus, respectively. The roles of genes possibly involved in post-core-assembly steps in herbicidin biosynthesis in these clusters and a related her cluster were studied. Through systematic gene deletions, structural elucidation of the accumulated intermediates in the mutants, and in vitro verification of the encoded enzymes, the peripheral modification pathway for herbicidin biosynthesis is now fully established.

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