2. Academic Validation
  3. Unusual isovalerylated flavonoids from the fruit of sea buckthorn (Elaeagnus rhamnoides) grown in Sokółka, Poland

Unusual isovalerylated flavonoids from the fruit of sea buckthorn (Elaeagnus rhamnoides) grown in Sokółka, Poland

  • Phytochemistry. 2019 Jul:163:178-186. doi: 10.1016/j.phytochem.2019.03.001.
Jerzy Żuchowski 1,  Łukasz Pecio 2,  Beata Marciniak 3,  Renata Kontek 4,  Anna Stochmal 5
Affiliations

Affiliations

  • 1 Department of Biochemistry and Crop Quality, Institute of Soil Science and Plant Cultivation - State Research Institute, ul. Czartoryskich 8, 24-100 Puławy, Poland. Electronic address: [email protected].
  • 2 Department of Biochemistry and Crop Quality, Institute of Soil Science and Plant Cultivation - State Research Institute, ul. Czartoryskich 8, 24-100 Puławy, Poland. Electronic address: [email protected].
  • 3 Laboratory of Cytogenetics, Faculty of Biology and Environmental Protection, University of Łódź, ul. Banacha 12/16, 90 237 Łódź, Poland. Electronic address: [email protected].
  • 4 Laboratory of Cytogenetics, Faculty of Biology and Environmental Protection, University of Łódź, ul. Banacha 12/16, 90 237 Łódź, Poland. Electronic address: [email protected].
  • 5 Department of Biochemistry and Crop Quality, Institute of Soil Science and Plant Cultivation - State Research Institute, ul. Czartoryskich 8, 24-100 Puławy, Poland. Electronic address: [email protected].
PMID: 30952449 DOI: 10.1016/j.phytochem.2019.03.001
Abstract

Eight undescribed isorhamnetin glycosides, acylated with isovaleric acid were isolated from the fruit of sea buckthorn (Elaeagnus rhamnoides (L.) A. Nelson). Structures of the purified compounds were determined using one and two-dimensional NMR spectroscopy, mass spectrometry and chemical methods. In addition, the cytotoxic activity of the phenolic-rich fraction of sea buckthorn fruit and its major Flavonoids against colon cell lines, HT-29, HCT-116 and Caco-2, was determined. While the phenolic fraction was moderately active against HT-29 and HCT-116, all investigated purified Flavonoids showed significantly weaker activity. This is most probably the first report about isorhamnetin glycosides acylated with isovaleric acid.

Keywords

3-methylbutanoic acid; Elaeagnaceae; Elaeagnus rhamnoides (L.) A. Nelson; Flavonoids; Fruit; Isorhamnetin; Isovaleric acid; Phenolic compounds; Sea buckthorn.

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