2. Academic Validation
  3. Phloretin loaded chitosan nanoparticles enhance the antioxidants and apoptotic mechanisms in DMBA induced experimental carcinogenesis

Phloretin loaded chitosan nanoparticles enhance the antioxidants and apoptotic mechanisms in DMBA induced experimental carcinogenesis

  • Chem Biol Interact. 2019 Aug 1:308:11-19. doi: 10.1016/j.cbi.2019.05.008.
Arokia Vijaya Anand Mariadoss 1,  Ramachandran Vinayagam 2,  Baojun Xu 3,  Karthikkumar Venkatachalam 4,  Vijayalakshmi Sankaran 2,  Shalini Vijayakumar 2,  Sandya Rani Bakthavatsalam 5,  Sadiq A Mohamed 6,  Ernest David 7
Affiliations

Affiliations

  • 1 Department of Biotechnology, Thiruvalluvar University, Serkadu, Vellore, 632 115, Tamilnadu, India. Electronic address: [email protected].
  • 2 Department of Biotechnology, Thiruvalluvar University, Serkadu, Vellore, 632 115, Tamilnadu, India.
  • 3 Food Science and Technology Program, Beijing Normal University-Hong Kong Baptist University, United International College, Zhuhai, 519087, China.
  • 4 Department of Pharmacology and Therapeutics, College of Medicine and Health Sciences, UAE University, Al Ain, United Arab Emirates.
  • 5 Center for Stem Cell Research, Christian Medical College, Vellore, 632004, India.
  • 6 Adhiparasakthi College of Arts and Science, G.B. Nagar, Kalavai, 632 506, Tamilnadu, India.
  • 7 Department of Biotechnology, Thiruvalluvar University, Serkadu, Vellore, 632 115, Tamilnadu, India. Electronic address: [email protected].
PMID: 31071336 DOI: 10.1016/j.cbi.2019.05.008
Abstract

The main aim of this study was to investigate the effects of phloretin loaded chitosan nanoparticles (PhCsNPs) on 7,12-dimethylbenz[a]anthracene (DMBA) induced experimental Cancer in hamsters. Oral squamous cell carcinoma (OSCC) was induced in male golden Syrian hamsters by painting with 0.5% DMBA three times a week for 14 weeks. Varying concentration of PhCsNPs (5, 10, and 20 mg/kg b.wt.) was orally administered on alternative days to evaluate the optimum dose. The experiment design was terminated at the end of the 14th week. The development of OSCC was confirmed by histopathological and biochemical analysis (lipid peroxidation, antioxidant profile, and detoxification Enzymes) in plasma, erythrocyte, buccal, and liver tissues. Significant increases in oxidation and lipid peroxidation were noticed in DMBA-painted hamsters. Oral administration of PhCsNPs in various doses on alternate days reversed the deleterious effects induced by DMBA. In addition, immunoblot analyses of PhCsNPs treatment enhanced the release of Bcl-2 associated X protein (Bax), cytochrome c, Caspase-3, 9 and suppressed the B-cell lymphoma 2 (Bcl-2) expression, which the use of PhCsNPs for mitochondrial-mediated Apoptosis. These findings suggest biofabricated PhCsNPs may act as a potent antioxidant and anti-carcinogenic in DMBA induced oral Cancer in experimental Animals.

Keywords

Antioxidants; Chitosan nanoparticles; DMBA; Oral cancer; Phloretin.

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