2. Academic Validation
  3. Chemical constituents from the seeds of Cassia obtusefolia and their in vitro α-glucosidase inhibitory and antioxidant activities

Chemical constituents from the seeds of Cassia obtusefolia and their in vitro α-glucosidase inhibitory and antioxidant activities

  • Bioorg Med Chem Lett. 2019 Jul 1;29(13):1576-1579. doi: 10.1016/j.bmcl.2019.05.002.
Han-Yan Luo 1,  Ri-Xin Guo 2,  Xian-Kuo Yu 1,  Xiao Zhang 3,  Ya-Qi Lu 3,  Hong-Wei Wu 1,  Li-Ying Tang 4,  Zhu-Ju Wang 5
Affiliations

Affiliations

  • 1 Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China.
  • 2 National Institutes for Food and Drug Control, Beijing 100050, China.
  • 3 Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China; College of Pharmacy, Henan University of Chinese Medicine, Zhengzhou 450046, China.
  • 4 Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China. Electronic address: [email protected].
  • 5 Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China. Electronic address: [email protected].
PMID: 31078411 DOI: 10.1016/j.bmcl.2019.05.002
Abstract

Basing on chromatographic separation techniques, fifteen aglycones (1-15), including two new anthraquinone aglycones (1, 2) and thirteen known compounds (3-15), were isolated from the small polar fraction of Cassia obtusefolia (petroleum ether extract). Structural elucidations were performed by 1D/2D NMR spectroscopy and mass spectrometry. The in vitro antioxidant and α-glucosidase inhibitory activities of these fifteen compounds were determined. Except compounds 12 (IC50 3.03 ± 0.31 μg/mL, stronger than ascorbic acid, which IC50 was 6.48 ± 2.30 μg/mL) and 13 (IC50 78.40 ± 2.39 μg/mL), the free radical scavenging capacities of Other compounds were weak. Compounds 4, 5, 6 and 13 exhibited inhibitory activities on α-glucosidase with IC50 values of 50.60 ± 1.10, 22.57 ± 0.07, 60.09 ± 1.40, and 80.01 ± 2.66 μg/mL separately, however, all the α-glucosidase inhibitory activities were weaker than positive control (acarbose).

Keywords

Anthraquinone aglycones; Antioxidant; Cassia obtusefolia; Chemical constituents; α-Glucosidase inhibitory.

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