2. Academic Validation
  3. The Difference in Cytotoxic Activity between Two Optical Isomers of Gelsemine from Gelsemium elegans Benth. on PC12 Cells

The Difference in Cytotoxic Activity between Two Optical Isomers of Gelsemine from Gelsemium elegans Benth. on PC12 Cells

  • Molecules. 2019 May 25;24(10):2004. doi: 10.3390/molecules24102004.
Li Lin 1,  Yan-Chun Liu 2 3,  Zhao-Ying Liu 4 5
Affiliations

Affiliations

  • 1 College of Veterinary Medicine, Hunan Agricultural University, Changsha 410128, China. [email protected].
  • 2 College of Veterinary Medicine, Hunan Agricultural University, Changsha 410128, China. [email protected].
  • 3 Hunan Engineering Technology Research Center of Veterinary Drugs, Hunan Agricultural University, Changsha 410128, China. [email protected].
  • 4 College of Veterinary Medicine, Hunan Agricultural University, Changsha 410128, China. [email protected].
  • 5 Hunan Engineering Technology Research Center of Veterinary Drugs, Hunan Agricultural University, Changsha 410128, China. [email protected].
PMID: 31130596 DOI: 10.3390/molecules24102004
Abstract

Two optical isomers, +/- gelsemine (1, 2), together with one known compound were isolated from the whole plant of G. elegans. The structures of the separated constituents were elucidated on 1D and 2D (1H-1H COSY, HMBC, HSQC) NMR spectroscopy and high-resolution mass spectrometry (HRMS). The isolated Alkaloids were tested in vitro for cytotoxic potential against PC12 cells by the MTT assay. As a result, (+) gelsemine (compound 1) exhibited cytotoxic activity against PC12 cells with an IC50 value of 31.59 μM, while (-) gelsemine (compound 2) was not cytotoxic.

Keywords

Gelsemium elegans; PC12 cells; gelsemine; koumine.

Figures
Products