2. Academic Validation
  3. Pyridine nucleotide-disulphide oxidoreductase domain 2 (PYROXD2): Role in mitochondrial function

Pyridine nucleotide-disulphide oxidoreductase domain 2 (PYROXD2): Role in mitochondrial function

  • Mitochondrion. 2019 Jul;47:114-124. doi: 10.1016/j.mito.2019.05.007.
Tao Wang 1,  Xiaoyuan Xie 2,  HuiLin Liu 2,  Feng Chen 3,  Jianhua Du 4,  XingZhi Wang 1,  XingYan Jiang 1,  Fang Yu 1,  Handong Fan 5
Affiliations

Affiliations

  • 1 Institute of Aging Research, School of Medicine, Hangzhou Normal University, Hangzhou 310036, China.
  • 2 The Third Affiliated Hospital, Sun Yat-sen University, Guangzhou, Guangdong 510630, China.
  • 3 Blood center of Zhejiang province, Hangzhou, Zhejiang 310052, China.
  • 4 Nanchang Institute of Science and Technology, Nanchang 330108, China.
  • 5 Institute of Aging Research, School of Medicine, Hangzhou Normal University, Hangzhou 310036, China. Electronic address: [email protected].
PMID: 31170524 DOI: 10.1016/j.mito.2019.05.007
Abstract

Pyridine Nucleotide-Disulphide Oxidoreductase Domain 2 (PYROXD2), a Hepatitis B virus X protein (HBx)-interacting protein, is significantly down-regulated in hepatocellular carcinoma (HCC), however its exact biological function remains unclear. The aim of this study is to investigate the subcellular localization and biological function of PYROXD2 in hepatic cells. The results showed that PYROXD2 was imported to the mitochondrial inner membrane/matrix by Tom40 and Tim23, but not Mia40. PYROXD2 151-230aa might be the mitochondrial targeting sequence. PYROXD2 interacted with complex IV subunit COX5B. Knockout of PYROXD2 decreased MMP, intracellular ROS, complex IV activity, cell proliferation, ATP content and mtDNA copy number, but increased mtROS levels and the number of immature mitochondria. In summary, our data illustrated that PYROXD2 localizes to the mitochondrial inner membrane/matrix, and it plays important roles in regulating mitochondrial function.

Keywords

COX5B; Complex IV; Mitochondria; PYROXD2.

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