Cellular Interleukin Enhancer-Binding Factor 2, ILF2, Inhibits Japanese Encephalitis Virus Replication In Vitro

Viruses. 2019 Jun 17;11(6):559. doi: 10.3390/v11060559.

Xiaofang Cui ¹ ², Ping Qian ³ ⁴ ⁵ ⁶, Tingting Rao ⁷ ⁸, Yanming Wei ⁹ ¹⁰, Fang Zhao ¹¹ ¹², Huawei Zhang ¹³ ¹⁴, Huanchun Chen ¹⁵ ¹⁶ ¹⁷ ¹⁸, Xiangmin Li ¹⁹ ²⁰ ²¹ ²²

Affiliations

1 State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China. [email protected].
2 Laboratory of Animal Virology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China. [email protected].
3 State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China. [email protected].
4 Laboratory of Animal Virology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China. [email protected].
5 Key Laboratory of Development of Veterinary Diagnostic Products, Ministry of Agriculture, Wuhan 430070, China. [email protected].
6 Key Laboratory of Preventive Veterinary Medicine in Hubei Province, The Cooperative Innovation Center for Sustainable Pig Production, Wuhan 430070, China. [email protected].
7 State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China. [email protected].
8 Laboratory of Animal Virology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China. [email protected].
9 State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China. [email protected].
10 Laboratory of Animal Virology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China. [email protected].
11 State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China. [email protected].
12 Laboratory of Animal Virology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China. [email protected].
13 State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China. [email protected].
14 Laboratory of Animal Virology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China. [email protected].
15 State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China. [email protected].
16 Laboratory of Animal Virology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China. [email protected].
17 Key Laboratory of Development of Veterinary Diagnostic Products, Ministry of Agriculture, Wuhan 430070, China. [email protected].
18 Key Laboratory of Preventive Veterinary Medicine in Hubei Province, The Cooperative Innovation Center for Sustainable Pig Production, Wuhan 430070, China. [email protected].
19 State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China. [email protected].
20 Laboratory of Animal Virology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China. [email protected].
21 Key Laboratory of Development of Veterinary Diagnostic Products, Ministry of Agriculture, Wuhan 430070, China. [email protected].
22 Key Laboratory of Preventive Veterinary Medicine in Hubei Province, The Cooperative Innovation Center for Sustainable Pig Production, Wuhan 430070, China. [email protected].

PMID: 31212927 DOI: 10.3390/v11060559

Abstract

Japanese encephalitis virus (JEV) is a zoonotic mosquito-borne Flavivirus which is the leading causative agent of viral encephalitis in endemic regions. JEV NS3 is a component of the viral replicase complex and is a multifunctional protein. In this study, interleukin enhancer-binding factor 2 (ILF2) is identified as a novel cellular protein interacting with NS3 through co-immunoprecipitation assay and LC-MS/MS. The expression of ILF2 is decreased in JEV-infected human embryonic kidney (293T) cells. The knockdown of endogenous ILF2 by special short hairpin RNA (shRNA) positively regulates JEV propagation, whereas the overexpression of ILF2 results in a significantly reduced JEV genome synthesis. Further analysis revealed that the knockdown of ILF2 positively regulates viral replication by JEV replicon system studies. These results suggest that ILF2 may act as a potential Antiviral agent against JEV Infection.

Keywords

Japanese encephalitis virus; antiviral activity; cellular interleukin enhancer-binding factor 2 (ILF2); viral RNA translation.

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