2. Academic Validation
  3. Zearalenone induces NLRP3-dependent pyroptosis via activation of NF-κB modulated by autophagy in INS-1 cells

Zearalenone induces NLRP3-dependent pyroptosis via activation of NF-κB modulated by autophagy in INS-1 cells

  • Toxicology. 2019 Dec 1;428:152304. doi: 10.1016/j.tox.2019.152304.
Xue Wang 1,  Liping Jiang 2,  Limin Shi 3,  Kun Yao 4,  Xiance Sun 5,  Guang Yang 3,  Lijie Jiang 6,  Cong Zhang 3,  Ningning Wang 3,  Hongying Zhang 7,  Yan Wang 8,  Xiaofang Liu 9
Affiliations

Affiliations

  • 1 Department of Nutrition and Food Safety, College of Public Health, Dalian Medical University, No. 9, West Segment of South lvshun Road, Dalian 116044, Liaoning, PR China; Department of Teaching Affairs, the Second Affiliated Hospital of Dalian Medical University, Dalian 116023, PR China.
  • 2 Experimental Teaching Center of Public Health, Dalian Medical University, 9 W Lvshun South Road, Dalian, 116044, PR China.
  • 3 Department of Nutrition and Food Safety, College of Public Health, Dalian Medical University, No. 9, West Segment of South lvshun Road, Dalian 116044, Liaoning, PR China.
  • 4 Department of Orthopedics, the Second Affiliated Hospital of Dalian Medical University, Dalian 116023, PR China.
  • 5 Department of Occupational and Environmental Health, College of Public Health, Dalian Medical University. No. 9, West Segment of South lvshun Road, Dalian, 116044, Liaoning, PR China.
  • 6 Department of Internal Medicine, The Afliated Zhong Shan Hospital of Dalian University, Dalian, 116001, Liaoning, PR China.
  • 7 Department of Pathology and Forensic Medicine, Dalian Medical University, 9 West Lvshun Southern Road, Dalian 116044, PR China.
  • 8 Department of endocrinology, the Second Hospital of Chaoyang, No. 26, Chaoyang street of the twin towers, Chaoyang, 122000, PR China. Electronic address: [email protected].
  • 9 Department of Nutrition and Food Safety, College of Public Health, Dalian Medical University, No. 9, West Segment of South lvshun Road, Dalian 116044, Liaoning, PR China. Electronic address: [email protected].
PMID: 31586597 DOI: 10.1016/j.tox.2019.152304
Abstract

Zearalenone (ZEA), one of the mycotoxins widely found in food and feed, can stimulate an inflammatory reaction. In the present study, we demonstrated that ZEA induced the activation of NLRP3 inflammasome even pyroptotic cell death in rat Insulinoma Cell Line (INS-1). Meanwhile, according to the results of western blot and TEM, the level of Autophagy was elevated by ZEA, which protected against the activation of NLRP3 inflammasome and inflammatory response caused by ZEA. Furthermore, we indicated that ZEA-induced NF-κB p65 activation contributed to the activation of the NLRP3 inflammasome, inflammatory response, and Pyroptosis in INS-1 cells, which were indicated by western blot and immunofluorescence, and the activation of NF-κB p65 induced by ZEA was autophagy-dependent. This study demonstrates that ZEA induces NLRP3-dependent Pyroptosis via activation of NF-κB modulated by Autophagy in INS-1 cells.

Keywords

Autophagy; NF-κB p65; NLRP3 inflammasome; Pyroptosis; Zearalenone.

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