2. Academic Validation
  3. Myocardial protection properties of parishins from the roots of Gastrodia elata Bl

Myocardial protection properties of parishins from the roots of Gastrodia elata Bl

  • Biomed Pharmacother. 2020 Jan:121:109645. doi: 10.1016/j.biopha.2019.109645.
Qi Wang 1,  Zhifeng Li 2,  Dongxu Wang 3,  Shinlin Yang 3,  Yulin Feng 4
Affiliations

Affiliations

  • 1 State Key Laboratory of Innovative Drug and Efficient Energy-Saving Pharmaceutical Equipment, Nanchang 330006, China.
  • 2 Jiangxi University of Traditional Chinese Medicine, Nanchang 330002, China. Electronic address: [email protected].
  • 3 Jiangxi University of Traditional Chinese Medicine, Nanchang 330002, China.
  • 4 State Key Laboratory of Innovative Drug and Efficient Energy-Saving Pharmaceutical Equipment, Nanchang 330006, China. Electronic address: [email protected].
PMID: 31739164 DOI: 10.1016/j.biopha.2019.109645
Abstract

Parishins, important constituents of Gastrodia elata (G. elata), are known to exhibit a number of biological and pharmacological properties. However, their role and mechanisms of action in myocardial ischemia are unknown. The present study investigated the potential protective effects and mechanisms of parishins extracted from G. elata on hypoxia/reoxygenation (H/R) injury in H9c2 cardiomyocytes. The results demonstrated that parishins had significant protective effects on myocardial cells with parishins J and B providing greater cardioprotection through down-regulation of the level of cleaved-caspase-3 and cytochrome c in the cytoplasm and Bax, and up-regulation of cytochrome c in the mitochondria and Bcl-2 than induced by the positive control gastrodin. Additional study of the mechanisms of action indicated that the myocardial protection provided by parishin J was due to inhibition of JNK1 phosphorylation levels, down-regulation of c-jun and ATF-2 phosphorylation levels, a decrease in the phosphorylation of 14-3-3 and an increase in its binding to Bax. Therefore, parishin J was revealed to be a promising candidate as a novel treatment for myocardial protection.

Keywords

Gastrodia elata; Myocardial ischemia; Parishins.

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