2. Academic Validation
  3. Genetic and Biochemical Characterization of an Exopolysaccharide With in vitro Antitumoral Activity Produced by Lactobacillus fermentum YL-11

Genetic and Biochemical Characterization of an Exopolysaccharide With in vitro Antitumoral Activity Produced by Lactobacillus fermentum YL-11

  • Front Microbiol. 2019 Dec 17;10:2898. doi: 10.3389/fmicb.2019.02898.
Yunlu Wei 1 2,  Fei Li 1 2,  Le Li 3,  Linlin Huang 1 2,  Quanhong Li 1 2
Affiliations

Affiliations

  • 1 College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, China.
  • 2 National Engineering Research Center for Fruit and Vegetable Processing, Beijing, China.
  • 3 Department of Environmental and Quality Inspection, Chongqing Chemical Industry Vocational College, Chongqing, China.
PMID: 31921073 DOI: 10.3389/fmicb.2019.02898
Abstract

In the present study, the whole genome sequence of Lactobacillus fermentum YL-11, a novel exopolysaccharide (EPS)-producing lactic acid bacteria (LAB) strain isolated from fermented milk, was determined. Genetic information and the synthetic mechanism of the EPS in L. fermentum YL-11 were identified based on bioinformatic analysis of the complete genome. The purified EPS of YL-11 mainly comprised galactose (48.0%), glucose (30.3%), mannose (11.8%), and arabinose (6.0%). In vitro, the EPS from YL-11 exhibited inhibition activity against HT-29 and Caco-2 colon Cancer cells, suggesting that EPS from strain YL-11 might be used as an antitumoral agent. EPS at 600 and 800 μg/mL achieved inhibition rates of 46.5 ± 3.5% and 45.6 ± 6.1% to HT-29 cells, respectively. The genomic information about L. fermentum YL-11 and the antitumoral activity of YL-11 EPS provide a theoretical foundation for the future application of EPS in the food and pharmaceutical industries.

Keywords

Lactobacillus fermentum; antitumoral activity; biosynthetic mechanism; exopolysaccharide; whole genome sequence.

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